EPPO - Database on Diagnostic Expertise

LIST OF VALIDATION DATA

Work in progress: Validation data from the previous version of the database will be transferred to this new version. Until this transfer is completed this information remains available via the link "Archives"
Downloading validation data in an excel file is possible for one organism at a time (see below).

The validation data on tests provided in this EPPO database represent the situation regarding these tests at the moment when the validation was performed and in the conditions of that validation.

Click here to access the template of the summary sheet of validation data for a diagnostic test.

Organism(s)	Matrix(ces)	Plant species tested	Detection / Identification	Methods	Kit	Follow EPPO diag	Follow IPPC diag	Data referred to EPPO	Report date	LabID
Erwinia amylovora	Leaves, Shoots	Rosaceae	detection	Extraction, Isolation		yes	no	yes	2012-03-01	IVIA-B
'Candidatus Phytoplasma mali', 'Candidatus Phytoplasma prunorum', 'Candidatus Phytoplasma pyri'	Leaves, Roots, Specimen, Wood, Woody cuttings	Malus domestica, Prunus sp., Pyrus communis	detection	Molecular Extraction DNA RNA, Molecular real time PCR	no	no	no	yes	2014-05-28	NIB-FITO
Pantoea stewartii subsp. stewartii	Other	Zea mays	detection and identification	Serological IF	yes	yes		yes	2011-05-31	EUPH-PANTOEA
Tomato spotted wilt virus	Leaves	Chrysanthemum sp., Solanum lycopersicum	detection and identification	Molecular Extraction DNA RNA, Molecular real time RT PCR, Serological DASI-ELISA	yes	yes	no	no	2018-02-12	ILVODGB
Meloidogyne chitwoodi, Meloidogyne fallax, Meloidogyne sp.	Soil, Tubers		detection and identification	Extraction, Morphological		yes		no	2008-12-31	ILVODGB
Xylella fastidiosa	Herbaceous cuttings	Brassica oleracea var. capitata, Vinca minor	detection	Extraction, Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	no, yes	no	no	2019-03-13	ISPP-CNR
Erwinia amylovora	Leaves, Other, Roots, Shoots	Rosaceae	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	yes	yes	yes	2010-01-01	AGES
Erwinia amylovora	Leaves, Shoots	Rosaceae	detection	Molecular Conventional PCR, Molecular Extraction DNA RNA	no, yes	yes	yes		2012-03-01	IVIA-B
Erwinia amylovora	Fruits, Leaves, Woody cuttings	Chaenomeles sp., Cotoneaster sp., Crataegus sp., Cydonia oblonga, Malus domestica, Pyracantha sp., Pyrus pyraster, Sorbus sp.	detection	Extraction, Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	yes	yes	yes	2011-10-20	NIB-FITO
Xylella fastidiosa	Leaves		detection	Molecular real time PCR	no	yes			2018-06-30	ILVODGB
Anoplophora glabripennis, Bemisia tabaci, Liriomyza huidobrensis, Spodoptera eridania, Vespa crabro	Specimen		identification	Molecular other	no	yes	no	yes	2016-06-30	EUPH-DNA
Clavibacter michiganensis subsp. michiganensis, Ralstonia solanacearum, Xanthomonas axonopodis pv. begoniae, Xanthomonas axonopodis pv. dieffenbachiae, Xylella fastidiosa	Pure culture		identification	Molecular other	no	yes	no	yes	2016-06-30	EUPH-DNA
Ceratocystis platani, Lecanosticta acicola, Phytophthora ramorum, Stagonosporopsis chrysanthemi, Verticillium dahliae	Other		identification	Molecular other	no	yes	no	yes	2016-06-30	EUPH-DNA
Hydrocotyle ranunculoides, Hydrocotyle vulgaris, Ludwigia grandiflora, Ludwigia peploides, Myriophyllum heterophyllum	Specimen		identification	Molecular other	no	yes	no	yes	2016-06-30	EUPH-DNA
'Candidatus Phytoplasma mali', 'Candidatus Phytoplasma prunorum', 'Candidatus Phytoplasma pyri', 'Candidatus Phytoplasma solani'	Other		identification	Molecular other	no	yes	no	yes	2016-06-30	EUPH-DNA
Aphelenchoides besseyi, Aphelenchoides fragariae, Bursaphelenchus xylophilus, Ditylenchus dipsaci, Meloidogyne chitwoodi	Specimen		identification	Molecular other	no	yes	no	yes	2016-06-30	EUPH-DNA
Globodera pallida, Globodera rostochiensis	Other		detection and identification	Extraction, Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	yes	no	no	2013-09-01	Fera
Pseudomonas syringae pv. actinidiae	Bark, Fruits, Leaves, Other, Pure culture	Actinidia chinensis	detection	Extraction, Fingerprint, Isolation, Molecular Conventional PCR, Molecular Extraction DNA RNA, Morphological		yes	no	yes	2014-06-05	CREA-DC-Roma
Nepovirus	Leaves	Ajuga sp., Chenopodium quinoa, Cucumis sativus, Nicotiana benthamiana, Nicotiana clevelandii, Prunus avium, Prunus persica, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera		Molecular Conventional RT PCR, Molecular Extraction DNA RNA	yes	no	no	no	2015-11-01	LNPVAAL
'Candidatus Phytoplasma mali', 'Candidatus Phytoplasma prunorum', 'Candidatus Phytoplasma pyri'	Woody cuttings	Malus sp., Prunus sp., Pyrus sp.		Molecular Extraction DNA RNA, Molecular PCR-RFLP		yes		yes	2013-01-01	LNPVAAL
Xanthomonas axonopodis pv. dieffenbachiae	Leaves, Pure culture	Anthurium	detection	Extraction, Serological IF		yes		no	2012-03-01	LNPVRAPT
Xanthomonas axonopodis pv. dieffenbachiae	Leaves, Pure culture	Anthurium	detection	Extraction, Molecular Conventional PCR, Molecular PCR-RFLP		yes		no	2012-03-01	LNPVRAPT
Xanthomonas axonopodis pv. dieffenbachiae	Leaves, Pure culture	Anthurium	detection	Extraction, Serological DAS-ELISA		yes		no	2012-03-01	LNPVRAPT
'Candidatus Phytoplasma mali'	Leaves	Malus sp., Prunus sp., Pyrus sp.	detection	Molecular Extraction DNA RNA, Molecular real time PCR	yes	no	no	no	2013-01-01	CREA-DC-Roma
'Candidatus Phytoplasma mali'	Leaves	Malus sp., Prunus sp., Pyrus sp.	detection	Molecular Conventional PCR, Molecular Extraction DNA RNA	yes	no, yes		no	2013-01-01	CREA-DC-Roma
'Candidatus Phytoplasma mali'	Leaves	Malus sp., Prunus sp., Pyrus sp.	detection	Molecular Conventional PCR, Molecular Extraction DNA RNA	yes	no	no	no	2013-01-01	CREA-DC-Roma
'Candidatus Phytoplasma prunorum'	Bark, Leaves	Malus domestica, Prunus armeniaca, Prunus domestica, Prunus persica, Pyrus communis	detection	Molecular Extraction DNA RNA, Molecular real time PCR	yes	no	no	no	2013-01-01	CREA-DC-Roma
'Candidatus Phytoplasma prunorum'	Bark, Leaves	Malus domestica, Prunus armeniaca, Prunus domestica, Prunus persica, Pyrus communis	detection	Molecular Conventional PCR, Molecular Extraction DNA RNA	yes	no	no	no	2013-01-01	CREA-DC-Roma
Plum pox virus	Bark, Leaves	Nicotiana benthamiana, Prunus armeniaca, Prunus domestica, Prunus persica, Terminalia catappa	detection	Extraction, Serological DAS-ELISA, Serological DASI-ELISA	yes	yes		no	2013-01-01	CREA-DC-Roma
Xylella fastidiosa	Leaves, Shoots	Asparagus acutifolius, Coffea, Lavandula, Nerium oleander, Olea europaea, Polygala myrtifolia, Quercus cerris, Salvia rosmarinus, Spartium junceum	detection	Molecular Extraction DNA RNA, Molecular real time PCR	yes	yes	no	yes	2016-02-26	NIB-FITO
Beet necrotic yellow vein virus	Roots	Beta vulgaris, Chenopodium quinoa, Nicotiana benthamiana, Spinacia oleracea	detection	Serological DAS-ELISA		yes		no	2014-11-01	LNPVAAL
Chrysanthemum stem necrosis virus	Leaves, Other	Chrysanthemum sp.		Serological DAS-ELISA	yes			no	2018-02-12	ILVODGB
Acidovorax citrulli	Pure culture, Seeds	Citrullus lanatus, Cucumis melo, Cucurbitaceae		Molecular Extraction DNA RNA, Molecular real time PCR	yes	yes		yes	2015-11-16	NAKTUINBOUW
Phyllosticta citricarpa	Fruits, Other	Citrus limon	detection	Molecular Conventional PCR, Molecular Extraction DNA RNA	yes	yes		no	2014-09-15	CREA-DC-Roma
Phyllosticta citricarpa	Fruits, Pure culture	Citrus limon		Molecular Extraction DNA RNA, Molecular real time PCR	yes	yes		no	2014-01-01	CREA-DC-Roma
Cucumber vein yellowing virus, Cucurbit yellow stunting disorder virus	Leaves	Citrullus lanatus, Cucumis melo, Cucumis sativus, Cucurbita pepo, Solanum lycopersicum	detection	Molecular Extraction DNA RNA, Molecular real time RT PCR		no	no	no	2014-01-01	LNPVAAL
'Candidatus Liberibacter solanacearum'	Seeds	Daucus carota	detection	Extraction, Molecular real time PCR	yes	no, yes	no	no	2016-05-06	IVIA-B
'Candidatus Liberibacter solanacearum'	Seeds	Daucus carota	detection	Extraction, Molecular real time PCR	yes	no, yes	no	no	2016-05-02	IVIA-B
'Candidatus Liberibacter solanacearum'	Seeds	Daucus carota	detection and identification	Molecular Conventional PCR, Molecular Extraction DNA RNA, Molecular real time PCR	yes	no, yes	no	yes	2017-05-25	CREA-DC-Roma
'Candidatus Liberibacter solanacearum'	Leaves	Apium graveolens, Daucus carota, Nicotiana tabacum, Solanum tuberosum, Vinca sp.	detection	Extraction, Molecular real time PCR	yes	yes		no	2016-04-28	IVIA-B
Xanthomonas fragariae	Leaves, Pure culture, Roots	Fragaria sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	yes	no	no	no	2011-05-17	NPPO-NL
Hymenoscyphus fraxineus	Leaves, Other, Shoots	Fraxinus sp.	detection	Molecular real time PCR		no	no	no	2009-10-01	LNPVUMAF
Xylella fastidiosa	Leaves, Shoots	Acacia sp., Acer sp., Asparagus sp., Callistemon sp., Citrus sp., Coffea sp., Cytisus sp., Ficus sp., Ginkgo sp., Grevillea sp., Hebe sp., Hedera sp., Helichrysum sp., Heliotropium sp., Hydrangea sp., Juglans sp., Laurus sp., Lavandula sp., Lonicera sp., Morus sp., Myrtus sp., Nerium sp., Olea sp., Origanum sp., Polygala sp., Prunus sp., Quercus sp., Rhamnus sp., Rosa sp., Rubus sp., Salvia, Spartium sp., Vaccinium sp., Vinca sp., Vitis sp.	detection	Molecular Extraction DNA RNA, Molecular real time PCR	yes	yes		no	2018-09-17	NIB-FITO
Xylella fastidiosa	Leaves, Shoots	Acacia sp., Acer sp., Asparagus sp., Callistemon sp., Citrus sp., Coffea sp., Cytisus sp., Ficus sp., Ginkgo sp., Grevillea sp., Hebe sp., Hedera sp., Heliotropium sp., Hydrangea sp., Juglans sp., Laurus sp., Lavandula sp., Lonicera sp., Morus sp., Myrtus sp., Nerium sp., Olea sp., Origanum sp., Polygala sp., Prunus sp., Quercus sp., Rhamnus sp., Rosa sp., Rubus sp., Salvia, Spartium sp., Vinca sp., Vitis sp.	detection	Molecular Extraction DNA RNA, Molecular real time PCR	yes	no, yes	no	no	2018-06-14	NIB-FITO
Xylella fastidiosa	Leaves, Shoots	Acacia sp., Acer sp., Asparagus sp., Callistemon sp., Citrus sp., Coffea sp., Cytisus sp., Ficus sp., Ginkgo sp., Grevillea sp., Hebe sp., Hedera sp., Heliotropium sp., Hydrangea sp., Juglans sp., Laurus sp., Lavandula sp., Lonicera sp., Morus sp., Myrtus sp., Nerium sp., Olea sp., Origanum sp., Polygala sp., Prunus sp., Quercus sp., Rhamnus sp., Rosa sp., Rubus sp., Salvia, Spartium sp., Vinca sp., Vitis sp.	detection	Molecular Extraction DNA RNA, Molecular real time PCR	yes	yes		no	2018-06-14	NIB-FITO
Xylella fastidiosa	Shoots	Lavandula sp.	detection	Extraction, Molecular Extraction DNA RNA, Molecular real time PCR	yes	no, yes		no	2019-03-11	ISPP-CNR
Xylella fastidiosa	Leaves	Nerium oleander	detection	Extraction, Molecular Extraction DNA RNA, Molecular real time PCR	yes	no, yes		no	2019-03-13	ISPP-CNR
Nacobbus aberrans	Specimen		detection	Molecular Conventional PCR, Molecular Extraction DNA RNA		yes		no	2009-01-19	LNPVnema
Bursaphelenchus xylophilus	Wood	Pinus sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time RT PCR		no	no		2014-09-01	LNPVnema
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	yes	no	no	2020-07-10	LNPVRAPT
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	yes	no	no	2020-07-10	LNPVRAPT
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	yes	no	no	2020-07-10	LNPVRAPT
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	yes	no	no	2020-07-10	LNPVRAPT
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	yes	no	no	2020-07-10	LNPVRAPT
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	yes	no	no	2020-07-10	LNPVRAPT
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	yes	no	no	2020-07-10	LNPVRAPT
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	no, yes	yes	no	no	2020-07-10	LNPVRAPT
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	yes	no	no	no	2020-07-10	LNPVRAPT
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Extraction DNA RNA, Molecular real time PCR	yes	no	no	no	2020-07-10	LNPVRAPT
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Conventional PCR	no	no	no	no	2020-07-10	LNPVRAPT
'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus'	Leaves	Citrus sp.	detection and identification	Molecular Conventional PCR	no	no	no	no	2020-07-10	LNPVRAPT
Bursaphelenchus xylophilus	Other		identification	Molecular real time PCR	yes	no	no		2020-07-30	CLEAR

This project has received funding from the European Union's Horizon 2020 research
and innovation programme under grant agreement n. 773139. The content of this website represents the views of the author only and is his/her sole responsibility. It cannot be considered to reflect the views of the European Commission and/or the Research Executive Agency or any other body of the European Union. The European Commission and the Agency do not accept any responsibility for use that may be made of the information it contains.