LIST OF VALIDATION DATA


This section provides an inventory of validation data (as referred to in EPPO PM 7/98 Specific requirements for laboratories preparing accreditation for a plant pest diagnostic activity) for diagnostic tests for plant pests. The validation data are provided by laboratories and the EPPO Secretariat is not responsible for the content of the validation sheets.

The validation data on tests provided in this EPPO database represent the situation regarding these tests at the moment when the validation was performed and in the conditions of that validation.

Click here to download an excel file with validation data for one organism at a time.
Click here to access the template of the summary sheet of validation data for a diagnostic test.
Click here to know how to add new validation data for diagnostic tests in the database.



Id Organism(s) Matrix(ces) Plant species tested Detection / Identification Methods Kit Follow EPPO diag
(yes / no)
and test names		 Follow IPPC diag
(yes / no)
and test names		 Report date LabID PDF
19 Erwinia amylovora Leaves, Shoots Rosaceae detection Extraction, Isolation yes
Antioxidant maceration buffer (Gorris et al.
1996)
Isolation on CCT medium (Ishimaru & Klos
1984)		 no
2012-03-01 IVIA-B
21 'Candidatus Phytoplasma mali', 'Candidatus Phytoplasma prunorum', 'Candidatus Phytoplasma pyri' Leaves, Roots, Specimen, Wood, Woody cuttings Malus domestica, Prunus sp., Pyrus communis detection Molecular Extraction DNA RNA, Molecular real time PCR no no
no
2014-05-28 NIB-FITO
22 Pantoea stewartii subsp. stewartii Other Zea mays detection and identification Serological IF yes yes
Cell staining according to PM7/97
2011-05-31 EUPH-PANTOEA
25 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Chrysanthemum sp., Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR, Serological DASI-ELISA yes yes
Taqman real time fluorescent RT-PCR (Boonham et al. 2001)
Triple Antibody Sandwich TAS-ELISA (based on Clark & Adams
1997)		 no
2018-02-12 ILVODGB
26 Meloidogyne chitwoodi, Meloidogyne fallax, Meloidogyne sp. Soil, Tubers detection and identification Extraction, Morphological yes
Centrifugal flotation (from soil)
Morphological identification
2008-12-31 ILVODGB
27 Xylella fastidiosa Herbaceous cuttings Brassica oleracea var. capitata, Vinca minor detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no
2019-03-13 ISPP-CNR
41 Erwinia amylovora Leaves, Other, Roots, Shoots Rosaceae detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR (Gottsberger
2010)		 yes
Real-time PCR according to Gottsberger (2010)		 2010-01-01 AGES
42 Erwinia amylovora Leaves, Shoots Rosaceae detection Molecular Conventional PCR, Molecular Extraction DNA RNA no, yes yes
PCR (Stoger et al. 2006)		 yes
PCR according to Stöger et al. (2006)		 2012-03-01 IVIA-B
43 Erwinia amylovora Fruits, Leaves, Woody cuttings Chaenomeles sp., Cotoneaster sp., Crataegus sp., Cydonia oblonga, Malus domestica, Pyracantha sp., Pyrus pyraster, Sorbus sp. detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
PBS buffer
Real-time PCR (Pirc et al. 2009)		 yes
DNA extraction according to Llop et al. (1999)		 2011-10-20 NIB-FITO
44 Erwinia amylovora Pure culture detection Serological Lateral Flow Device yes yes
Lateral flow devices
2012-12-18 AGES
92 Phytophthora ramorum Leaves Aesculus, Camellia, Castanea sativa, Ceanothus, Laurus nobilis, Photinia x fraseri, Pieris, Prunus lusitanica, Quercus, Rhododendron ponticum, Tilia, Viburnum davidii detection Molecular real time PCR no no
yes
Real-time PCR of Schena et al. (2006) targeting P. ramorum		 2018-02-05 choiseul
93 Bursaphelenchus xylophilus Specimen identification Molecular Conventional PCR no no
yes
Matsunaga et al. 2004		 2019-12-10 LNPVnema
94 Bursaphelenchus xylophilus Specimen identification Molecular PCR-RFLP no yes
ITS RFLP PCR (Burgermeister et al.
2009)
2019-12-10 LNPVnema
95 Bursaphelenchus xylophilus Specimen, Wood Pinus sp. detection Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real time PCR protocol for direct detection on wood extracts (Anses-LSV
FR)		 no
2019-12-10 LNPVnema
96 Bursaphelenchus xylophilus Wood Pinus sp. detection Molecular real time PCR yes no
no
2019-12-10 LNPVnema
97 Bursaphelenchus xylophilus Specimen, Wood Pinus sp. detection Molecular Extraction DNA RNA, Molecular LAMP no, yes no, yes
no, yes
Kikuchi et al. 2009		 2019-12-10 LNPVnema
98 Bursaphelenchus xylophilus Other Botrytis cinerea identification Molecular Conventional PCR no no
yes
Matsunaga et al. 2004		 2019-12-10 LNPVnema
100 Bursaphelenchus xylophilus Other, Wood Botrytis cinerea, Pinus sp. detection Molecular real time PCR no yes
Real time PCR protocol for direct detection on wood extracts (Anses-LSV
FR)		 no
2019-12-10 LNPVnema
101 Bursaphelenchus xylophilus Wood Pinus sp. detection Molecular real time PCR yes no
no
2019-12-10 LNPVnema
102 Bursaphelenchus xylophilus Wood Pinus sp. detection Molecular LAMP no no
yes
Kikuchi et al. 2009		 2019-12-10 LNPVnema
113 Xylella fastidiosa Leaves detection Molecular real time PCR no yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)
2018-06-30 ILVODGB
114 Anoplophora glabripennis, Bemisia tabaci, Liriomyza huidobrensis, Spodoptera eridania, Vespa crabro Specimen identification Molecular other no yes
no
2016-06-30 EUPH-DNA
115 Clavibacter michiganensis, Ralstonia solanacearum, Xanthomonas axonopodis pv. begoniae, Xanthomonas axonopodis pv. dieffenbachiae, Xylella fastidiosa Pure culture identification Molecular other no yes
no
2016-06-30 EUPH-DNA
116 Ceratocystis platani, Lecanosticta acicola, Phytophthora ramorum, Stagonosporopsis chrysanthemi, Verticillium dahliae Other identification Molecular other no yes
no
2016-06-30 EUPH-DNA
117 Hydrocotyle ranunculoides, Hydrocotyle vulgaris, Ludwigia grandiflora, Ludwigia peploides, Myriophyllum heterophyllum Specimen identification Molecular other no yes
no
2016-06-30 EUPH-DNA
118 'Candidatus Phytoplasma mali', 'Candidatus Phytoplasma prunorum', 'Candidatus Phytoplasma pyri', 'Candidatus Phytoplasma solani' Other identification Molecular other no yes
no
2016-06-30 EUPH-DNA
119 Aphelenchoides besseyi, Aphelenchoides fragariae, Bursaphelenchus xylophilus, Ditylenchus dipsaci, Meloidogyne chitwoodi Specimen identification Molecular other no yes
no
2016-06-30 EUPH-DNA
121 Globodera pallida, Globodera rostochiensis Other detection and identification Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Extraction Wye washer
Taqman Real-time PCR (Fera)		 no
2013-09-01 Fera
123 Pseudomonas syringae pv. actinidiae Bark, Fruits, Leaves, Other, Pure culture Actinidia chinensis detection Extraction, Fingerprint, Isolation, Molecular Conventional PCR, Molecular Extraction DNA RNA, Morphological yes
Duplex PCR (according to Gallelli et al.
2011a)
Isolation on modified King’s B medium (KB)
Isolation on modified Nutrient Sucrose Agar (NSA)
Morphological identification
PCR targeting the 16S-23S rDNA ITS regions (according to Rees-George et al.
2010)
Rep-PCR BOX according to PM7/100
Rep-PCR ERIC according to PM7/100
Rep-PCR REP according to PM7/100		 no
2014-06-05 CREA-DC-Roma
124 Nepovirus Leaves Ajuga sp., Chenopodium quinoa, Cucumis sativus, Nicotiana benthamiana, Nicotiana clevelandii, Prunus avium, Prunus persica, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera detection Molecular Conventional RT PCR, Molecular Extraction DNA RNA yes no
no
2015-11-01 LNPVAAL
125 'Candidatus Phytoplasma mali', 'Candidatus Phytoplasma prunorum', 'Candidatus Phytoplasma pyri' Woody cuttings Malus sp., Prunus sp., Pyrus sp. detection Molecular Extraction DNA RNA, Molecular PCR-RFLP yes
Conventional PCR for the generic detection of phytoplasmas (Lorenz et al.
1995) + RFLP (Schneider et al. (1995))
2013-01-01 LNPVAAL
126 Xanthomonas axonopodis pv. dieffenbachiae Leaves, Pure culture Anthurium detection Extraction, Serological IF yes
Extraction from symptomatic plant material in PBS buffer (Appendix 1.1)
2012-03-01 LNPVRAPT
127 Xanthomonas axonopodis pv. dieffenbachiae Leaves, Pure culture Anthurium detection Extraction, Molecular Conventional PCR, Molecular PCR-RFLP yes
Extraction from symptomatic plant material in PBS buffer (Appendix 1.1)
Nested PCR (Robene-Soustrade et al.
2006)
Nested PCR (Robene-Soustrade et al.
2006) + RFLP
2012-03-01 LNPVRAPT
128 Xanthomonas axonopodis pv. dieffenbachiae Leaves, Pure culture Anthurium detection Extraction, Serological DAS-ELISA yes
DAS-ELISA
Extraction from symptomatic plant material in PBS buffer (Appendix 1.1)
2012-03-01 LNPVRAPT
129 'Candidatus Phytoplasma mali' Leaves Malus sp., Prunus sp., Pyrus sp. detection Molecular Extraction DNA RNA, Molecular real time PCR yes no
no
2013-01-01 CREA-DC-Roma
130 'Candidatus Phytoplasma mali' Leaves Malus sp., Prunus sp., Pyrus sp. detection Molecular Conventional PCR, Molecular Extraction DNA RNA yes no, yes
AP group-specific nested PCR (adapted from Deng & Hiruki
1991; Schneider et al.
1995; Lorenz et al.
1995)
2013-01-01 CREA-DC-Roma
131 'Candidatus Phytoplasma mali' Leaves Malus sp., Prunus sp., Pyrus sp. detection Molecular Conventional PCR, Molecular Extraction DNA RNA yes no
no
2013-01-01 CREA-DC-Roma
132 'Candidatus Phytoplasma prunorum' Bark, Leaves Malus domestica, Prunus armeniaca, Prunus domestica, Prunus persica, Pyrus communis detection Molecular Extraction DNA RNA, Molecular real time PCR yes no
no
2013-01-01 CREA-DC-Roma
133 'Candidatus Phytoplasma prunorum' Bark, Leaves Malus domestica, Prunus armeniaca, Prunus domestica, Prunus persica, Pyrus communis detection Molecular Conventional PCR, Molecular Extraction DNA RNA yes no
no
2013-01-01 CREA-DC-Roma
134 Potyvirus plumpoxi / Plum pox virus Bark, Leaves Nicotiana benthamiana, Prunus armeniaca, Prunus domestica, Prunus persica, Terminalia catappa detection Extraction, Serological DAS-ELISA, Serological DASI-ELISA yes yes
DASI-ELISA (Cambra et al.
1994)
2013-01-01 CREA-DC-Roma
135 Xylella fastidiosa Leaves, Shoots Asparagus acutifolius, Coffea, Lavandula, Nerium oleander, Olea europaea, Polygala myrtifolia, Quercus cerris, Salvia rosmarinus, Spartium junceum detection Molecular Extraction DNA RNA, Molecular real time PCR yes yes
Taqman real-time PCR tests (based on Francis et al.
2006)		 no
2016-02-26 NIB-FITO
136 Benyvirus necrobetae / Beet necrotic yellow vein virus Roots Beta vulgaris, Chenopodium quinoa, Nicotiana benthamiana, Spinacia oleracea detection Serological DAS-ELISA yes

2014-11-01 LNPVAAL
137 Orthotospovirus chrysanthinecrocaulis / Chrysanthemum stem necrosis virus Leaves, Other Chrysanthemum sp. detection Serological DAS-ELISA yes

2018-02-12 ILVODGB
138 Acidovorax citrulli Pure culture, Seeds Citrullus lanatus, Cucumis melo, Cucurbitaceae detection Molecular Extraction DNA RNA, Molecular real time PCR yes yes
Real-time PCR targeting the IS1002 element (Woudt et al.
2009a
b) and Contig 22
2015-11-16 NAKTUINBOUW
139 Phyllosticta citricarpa Fruits, Other Citrus x limon detection Molecular Conventional PCR, Molecular Extraction DNA RNA yes yes
Conventional PCR (Bonants et al.
2003)
2014-09-15 CREA-DC-Roma
140 Phyllosticta citricarpa Fruits, Pure culture Citrus x limon identification Molecular Extraction DNA RNA, Molecular real time PCR yes yes
Real-time PCR (van Gent-Pelzer et al.
2007)
2014-01-01 CREA-DC-Roma
141 Crinivirus cucurbitae, Ipomovirus cucumisvenaflavi / Cucurbit yellow stunting disorder virus Leaves Citrullus lanatus, Cucumis melo, Cucumis sativus, Cucurbita pepo, Solanum lycopersicum detection Molecular Extraction DNA RNA, Molecular real time RT PCR no
no
2014-01-01 LNPVAAL
142 'Candidatus Liberibacter solanacearum' Seeds Daucus carota detection Extraction, Molecular real time PCR yes no, yes
Real-time PCR based on 16S rRNA gene (Teresani et al.
2014)		 no
2016-05-06 IVIA-B
143 'Candidatus Liberibacter solanacearum' Seeds Daucus carota detection Extraction, Molecular real time PCR yes no, yes
Real-time PCR based on 16S rRNA gene (Teresani et al.
2014)		 no
2016-05-02 IVIA-B
144 'Candidatus Liberibacter solanacearum' Seeds Daucus carota detection and identification Molecular Conventional PCR, Molecular Extraction DNA RNA, Molecular real time PCR yes no, yes
Conventional end-point PCR (Ravindran et al.
2011)
Real-time PCR adapted by ANSES from Li et al. 2009		 no
2017-05-25 CREA-DC-Roma
145 'Candidatus Liberibacter solanacearum' Leaves Apium graveolens, Daucus carota, Nicotiana tabacum, Solanum tuberosum, Vinca sp. detection Extraction, Molecular real time PCR yes yes
Real-time PCR based on 16S rRNA gene (Teresani et al.
2014)
2016-04-28 IVIA-B
146 Xanthomonas fragariae Leaves, Pure culture, Roots Fragaria sp. detection and identification Molecular Extraction DNA RNA, Molecular real time PCR yes no
no
2011-05-17 NIVIP
147 Hymenoscyphus fraxineus Leaves, Other, Shoots Fraxinus sp. detection Molecular real time PCR no
no
2009-10-01 LNPVUMAF
148 Xylella fastidiosa Leaves, Shoots Acacia sp., Acer sp., Asparagus sp., Callistemon sp., Citrus sp., Coffea sp., Cytisus sp., Ficus sp., Ginkgo sp., Grevillea sp., Hedera sp., Helichrysum sp., Heliotropium sp., Hydrangea sp., Juglans sp., Laurus sp., Lavandula sp., Lonicera sp., Morus sp., Myrtus sp., Nerium sp., Olea sp., Origanum sp., Polygala sp., Prunus sp., Quercus sp., Rhamnus sp., Rosa sp., Rubus sp., Salvia, Spartium sp., Vaccinium sp., Vinca sp., Vitis sp. detection Molecular Extraction DNA RNA, Molecular real time PCR yes yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)
2018-09-17 NIB-FITO
149 Xylella fastidiosa Leaves, Shoots Acacia sp., Acer sp., Asparagus sp., Callistemon sp., Citrus sp., Coffea sp., Cytisus sp., Ficus sp., Ginkgo sp., Grevillea sp., Hedera sp., Heliotropium sp., Hydrangea sp., Juglans sp., Laurus sp., Lavandula sp., Lonicera sp., Morus sp., Myrtus sp., Nerium sp., Olea sp., Origanum sp., Polygala sp., Prunus sp., Quercus sp., Rhamnus sp., Rosa sp., Rubus sp., Salvia, Spartium sp., Vinca sp., Vitis sp. detection Molecular Extraction DNA RNA, Molecular real time PCR yes no, yes
no
2018-06-14 NIB-FITO
150 Xylella fastidiosa Leaves, Shoots Acacia sp., Acer sp., Asparagus sp., Callistemon sp., Citrus sp., Coffea sp., Cytisus sp., Ficus sp., Ginkgo sp., Grevillea sp., Hedera sp., Heliotropium sp., Hydrangea sp., Juglans sp., Laurus sp., Lavandula sp., Lonicera sp., Morus sp., Myrtus sp., Nerium sp., Olea sp., Origanum sp., Polygala sp., Prunus sp., Quercus sp., Rhamnus sp., Rosa sp., Rubus sp., Salvia, Spartium sp., Vinca sp., Vitis sp. detection Molecular Extraction DNA RNA, Molecular real time PCR yes yes
Taqman real-time PCR tests (based on Francis et al.
2006)
2018-06-14 NIB-FITO
151 Xylella fastidiosa Shoots Lavandula sp. detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)
2019-03-11 ISPP-CNR
152 Xylella fastidiosa Leaves Nerium oleander detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)
2019-03-13 ISPP-CNR
153 Aphelenchoides besseyi Specimen detection and identification Molecular real time PCR yes yes
Real-time PCR test based on SSU rDNA (Clear detection)
2011-01-01 CLEAR
154 Meloidogyne chitwoodi, Meloidogyne fallax Specimen detection and identification Molecular real time PCR yes yes
Real-time SYBR-green PCR (LSU rDNA based Test (ClearDectections))
2013-08-01 CLEAR
155 Globodera pallida, Globodera rostochiensis Specimen detection and identification Molecular real time PCR yes yes

2013-08-01 CLEAR
156 Bursaphelenchus xylophilus Specimen identification Morphological yes

2011-05-01 LNPVnema
157 Diabrotica virgifera virgifera Specimen identification Extraction, Morphological yes

2011-05-19 ILVODGB
158 Thrips palmi Specimen identification Extraction, Morphological yes

2011-05-19 ILVODGB
161 Xylella fastidiosa Leaves Nerium oleander, Olea europaea detection Extraction, Molecular LAMP

2015-07-01 ISPP-CNR
162 Xylella fastidiosa Leaves Acacia saligna, Citrus sp., Nerium oleander, Olea europaea, Polygala myrtifolia, Prunus avium, Prunus dulcis, Quercus ilex, Vitis sp. detection Molecular Extraction DNA RNA, Molecular real time PCR no
no
2014-09-01 ISPP-CNR
163 Xylella fastidiosa Leaves Acacia saligna, Citrus sp., Nerium oleander, Olea europaea, Polygala myrtifolia, Prunus avium, Prunus dulcis, Quercus ilex, Vitis sp. detection Serological DAS-ELISA yes no

2014-09-01 ISPP-CNR
164 Xylella fastidiosa Leaves Acacia saligna, Citrus sp., Nerium oleander, Olea europaea, Polygala myrtifolia, Prunus avium, Prunus dulcis, Quercus ilex, Vitis sp. detection Molecular Conventional PCR, Molecular Extraction DNA RNA yes
Conventional PCR (Minsavage et al.
1994)
2014-09-01 ISPP-CNR
165 Xylella fastidiosa Leaves Nerium oleander, Olea europaea, Prunus avium, Prunus dulcis detection Molecular Extraction DNA RNA, Molecular real time PCR yes

2015-10-22 ISPP-CNR
166 Xylella fastidiosa Leaves Acacia saligna, Citrus sp., Nerium oleander, Olea europaea, Polygala myrtifolia, Prunus avium, Prunus dulcis, Quercus ilex, Vitis sp. detection Molecular Extraction DNA RNA, Molecular real time PCR yes no
no
2015-10-22 ISPP-CNR
167 Xylella fastidiosa Leaves Olea europaea, Quercus ilex detection Molecular Extraction DNA RNA, Molecular real time PCR yes yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)
2019-01-01 LNPVAAL
168 Xylella fastidiosa, Xylella fastidiosa subsp. pauca Leaves Olea europaea detection Extraction, Serological DAS-ELISA yes no
no
2015-10-28 CREA-DC-Roma
169 Xylella fastidiosa Leaves Olea europaea detection Molecular Conventional PCR, Molecular Extraction DNA RNA no, yes
Conventional PCR (Minsavage et al.
1994)		 no
2015-10-28 CREA-DC-Roma
170 Xylella fastidiosa subsp. pauca Leaves Olea europaea detection Molecular Extraction DNA RNA, Molecular real time PCR no, yes
no
2015-10-28 CREA-DC-Roma
171 Xylella fastidiosa subsp. pauca Leaves Olea europaea detection Extraction, Molecular LAMP yes no
no
2021-05-04 CREA-DC-Roma
172 Xylella fastidiosa subsp. pauca Leaves Olea europaea detection Molecular Extraction DNA RNA, Molecular LAMP yes

2015-10-28 CREA-DC-Roma
173 Xylella fastidiosa Leaves Olea europaea detection Molecular Extraction DNA RNA, Molecular real time PCR yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no
2021-09-26 ISPP-CNR
174 Potyvirus plumpoxi / Plum pox virus Leaves, Shoots Prunus armeniaca, Prunus cerasifera, Prunus domestica, Prunus persica detection Molecular Extraction DNA RNA, Molecular other yes

2011-01-01 FGU VNIIKR
175 Monilinia fructicola Fruits, Pure culture Malus domestica, Prunus domestica, Prunus persica, Pyrus communis detection Molecular Conventional PCR no
no
2013-04-06 CREA-DC-Roma
176 Potyvirus plumpoxi / Plum pox virus Leaves Prunus armeniaca, Prunus domestica, Prunus persica detection Extraction, Molecular Extraction DNA RNA, Molecular LAMP yes no
no
2014-01-01 CREA-DC-Roma
177 Ralstonia solanacearum species complex Shoots Pelargonium zonale detection Serological IF no yes

2011-10-13 NIVIP
178 Xylella fastidiosa Shoots Solanum lycopersicum, Vinca minor detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no
2019-03-13 ISPP-CNR
179 Xylella fastidiosa Shoots Polygala myrtifolia detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)
2019-03-13 ISPP-CNR
180 Xylella fastidiosa Shoots Prunus avium detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)
2019-03-13 ISPP-CNR
181 Monilinia fructicola Fruits Prunus sp. detection Molecular real time PCR yes
Real-time PCR (van Brouwershaven et al.)
2010-12-07 NIVIP
182 Potyvirus plumpoxi / Plum pox virus Leaves Prunus sp. detection Molecular real time RT PCR, Serological DAS-ELISA yes no, yes
yes
One-step real-time PCR according to Schneider et al (2004)		 2007-01-01 ILVODGB
183 Monilinia fructicola Flowers, Fruits, Other Malus sp., Prunus sp., Pyrus sp. detection Molecular Conventional PCR no
no
2008-01-01 LNPVUMAF
184 Phytophthora ramorum Leaves, Shoots Camellia japonica, Rhododendron ponticum, Viburnum x bodnantense detection Isolation, Morphological yes
Isolation from plant material with P5ARP [H]
2009-03-31 ILVODGB
185 Phytophthora kernoviae Leaves, Shoots Rhododendron ponticum detection Isolation, Morphological yes
Isolation on P5ARP [H] (Jeffers & Martin
1986)
Morphological identification P5ARP [H]
2009-12-21 ILVODGB
186 Xanthomonas euvesicatoria pv. euvesicatoria, Xanthomonas euvesicatoria pv. perforans, Xanthomonas hortorum pv. gardneri, Xanthomonas vesicatoria Seeds Capsicum annuum, Solanum lycopersicum detection Isolation yes
Isolation from seed on mMXV
Isolation from seed on mTMB		 no
2012-01-26 NAKTUINBOUW
187 Erwinia amylovora Leaves, Shoots Rosaceae detection Serological Lateral Flow Device yes yes
Lateral flow devices
2012-03-01 IVIA-B
188 Erwinia amylovora Leaves, Shoots Rosaceae detection Serological Lateral Flow Device yes yes
Lateral flow devices
2012-03-01 IVIA-B
189 Erwinia amylovora Leaves, Shoots Rosaceae detection Molecular Conventional PCR, Molecular Extraction DNA RNA yes yes
PCR (Gottsberger adapted from Obradovic et al. 2007)
2012-03-01 IVIA-B
190 Erwinia amylovora Leaves, Shoots Rosaceae detection Molecular Conventional PCR, Molecular Extraction DNA RNA yes yes
PCR (Taylor et al. 2001)
2012-03-01 IVIA-B
191 Erwinia amylovora Leaves, Shoots Rosaceae detection Molecular Extraction DNA RNA, Molecular real time PCR yes yes
Real-time PCR (Pirc et al. 2009)
2012-03-01 IVIA-B
192 Erwinia amylovora Leaves, Shoots Rosaceae detection Molecular Extraction DNA RNA, Molecular LAMP yes
LAMP (Temple et al. 2008 and Temple & Johnson 2011)
2012-03-01 IVIA-B
193 Erwinia amylovora Leaves, Shoots Rosaceae detection Serological DASI-ELISA yes
enrichment DASI-ELISA (Gorris et al. 1996)
2012-03-01 IVIA-B
194 Erwinia amylovora Leaves, Shoots Rosaceae detection Serological IF yes
Immunofluorescence (IF)
2012-03-01 IVIA-B
195 Erwinia amylovora Leaves, Shoots Rosaceae detection Isolation yes
Isolation on King's B medium (King et al.
1954)
2012-03-01 IVIA-B
196 Erwinia amylovora Leaves, Shoots Rosaceae detection Molecular Extraction DNA RNA, Molecular real time PCR yes no, yes
Real-time PCR (Gottsberger
2010)		 no
2014-01-01 FGU VNIIKR
197 Erwinia amylovora Leaves, Shoots Rosaceae detection Molecular Conventional PCR, Molecular Extraction DNA RNA yes yes
PCR (Stoger et al. 2006)
2014-01-01 FGU VNIIKR
198 Crinivirus tomatichlorosis Leaves Solanum tuberosum detection Extraction, Molecular Conventional RT PCR, Molecular Extraction DNA RNA yes no
no
2011-07-01 LNPVAAL
199 Potexvirus pepini / Pepino mosaic virus Leaves, Seeds Solanum lycopersicum detection Extraction, Molecular Extraction DNA RNA, Molecular real time RT PCR yes no
no
2012-10-01 LNPVAAL
200 Clavibacter michiganensis Leaves, Shoots Solanum lycopersicum detection Isolation yes
Dilution plating on semi selective media (CMMIT
SCMF or SCM) - plant tissue		 no
2010-04-01 NIVIP
201 Clavibacter michiganensis Seeds Solanum lycopersicum detection Isolation yes
Dilution plating on semi selective media (CMMIT and SCMF or SCM) - seeds		 no
2010-02-02 NIVIP
202 Pospiviroid chloronani, Pospiviroid fusituberis / Potato spindle tuber viroid, Tomato chlorotic dwarf viroid Leaves Solanum lycopersicum detection and identification Molecular Conventional RT PCR, Molecular Extraction DNA RNA, Molecular other yes
yes
Conventional RT-PCR using the primers of Shamloul et al. (1997)		 2013-09-17 NIVIP
203 Pospiviroid chloronani, Pospiviroid fusituberis, Pospiviroid latenscolumneae / Columnea latent viroid, Potato spindle tuber viroid, Tomato chlorotic dwarf viroid Leaves Solanum lycopersicum detection and identification Molecular Conventional RT PCR, Molecular Extraction DNA RNA, Molecular other yes
yes
Conventional RT-PCR using the primers of Verhoeven et al. (2004)		 2013-09-17 NIVIP
204 Pospiviroid Leaves Solanum lycopersicum detection Molecular Extraction DNA RNA, Molecular real time RT PCR yes no
no
2012-04-04 NIVIP
205 Pospiviroid chloronani, Pospiviroid fusituberis / Potato spindle tuber viroid, Tomato chlorotic dwarf viroid Seeds Solanum lycopersicum detection Molecular Extraction DNA RNA, Molecular real time RT PCR yes no
no, yes
Real-time RT-PCR using the primers of Boonham et al. (2004)		 2012-05-02 NAKTUINBOUW
206 Pospiviroid Leaves, Seeds Chrysanthemum sp., Solanum lycopersicum detection Molecular real time RT PCR no
no
2018-02-12 ILVODGB
207 Pospiviroid Seeds Capsicum annuum, Solanum lycopersicum detection Extraction, Molecular Extraction DNA RNA, Molecular real time RT PCR yes yes
Real-time RT-PCR for seed testing (Naktuinbouw
2017)
Seeds - Homogenization in GH+ buffer		 no
2015-10-08 NAKTUINBOUW
208 Pospiviroid, Pospiviroid fusituberis / Potato spindle tuber viroid Leaves Celosia sp., Solanum lycopersicum detection and identification Molecular Conventional RT PCR, Molecular Extraction DNA RNA, Molecular other yes
yes
Conventional RT-PCR using the primers of Verhoeven et al. (2004)		 2013-09-17 NIVIP
209 Orthotospovirus chrysanthinecrocaulis, Orthotospovirus impatiensnecromaculae, Orthotospovirus tomatomaculae / Chrysanthemum stem necrosis virus, Impatiens necrotic spot virus, Tomato spotted wilt virus Leaves Allium sp., Capsicum sp., Chrysanthemum sp., Cineraria sp., Nicotiana benthamiana, Solanum lycopersicum detection Molecular Conventional RT PCR, Molecular Extraction DNA RNA yes no
no
2015-01-01 LNPVAAL
210 Pospiviroid Leaves Brugmansia sp., Calibrachoa sp., Cestrum sp., Dahlia sp., Lycianthes sp., Nematanthus sp., Petunia sp., Solanum laxum, Solanum lycopersicum, Streptosolen sp., Vinca sp. detection Molecular Extraction DNA RNA, Molecular real time RT PCR yes yes
Real-time RT-PCR (Botermans et al.
2013)		 no
2012-09-28 NAKTUINBOUW
211 Pospiviroid chloronani, Pospiviroid fusituberis / Potato spindle tuber viroid, Tomato chlorotic dwarf viroid Leaves Brugmansia sp., Calibrachoa sp., Dahlia sp., Lycianthes sp., Petunia sp., Solanum laxum, Solanum lycopersicum, Streptosolen sp. detection Molecular Extraction DNA RNA, Molecular real time RT PCR yes no
yes
Real-time RT-PCR using the primers of Boonham et al. (2004)		 2012-08-28 NAKTUINBOUW
212 Pospiviroid Leaves Solanum tuberosum detection Molecular real time RT PCR no
no
2011-11-01 NAK
213 Pospiviroid fusituberis / Potato spindle tuber viroid Leaves Solanum tuberosum detection Molecular real time RT PCR yes
Taqman real time RT PCR
2015-01-01 NAK
214 Meloidogyne chitwoodi, Meloidogyne fallax Tubers Solanum tuberosum detection Molecular real time PCR yes
TaqMan ITS based real-time PCR (Zijlsta & van Hoof
2006)
2008-01-01 NAK
215 'Candidatus Liberibacter solanacearum' Tubers Solanum tuberosum detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR yes yes
no
2018-04-03 EVIRAPQL
216 'Candidatus Liberibacter solanacearum' Leaves, Roots Daucus carota, Solanum tuberosum detection Molecular Extraction DNA RNA, Molecular real time PCR yes
yes
Li et al. 2009 Li W.
Abad J.A.
French-Monar R.D.
Rascoe J.
Wen A.
Gudmestad N.C.
Secor G.A.
Lee I-M.
Duan Y.
Levy L. Multiplex real-time PCR for detection
identification and quantification of ‘Candi-datus Liberibacter solanacearum’ in potato plants with zebra chip. Journal of Microbiological Meth-ods 78
59–65 (2009).		 2016-06-28 ILVODGB
217 Ralstonia solanacearum species complex Leaves, Tubers, Water Pelargonium sp., Rosa sp., Solanum tuberosum detection Isolation

2009-09-01 NIVIP
218 Ralstonia solanacearum species complex Shoots, Tubers Solanum lycopersicum, Solanum tuberosum detection Molecular LAMP

2017-02-09 NIB-FITO
219 Grapevine flavescence dorée phytoplasma Other Vitis sp. detection Molecular real time PCR no
no
2014-07-31 EUPH-GRAFDEPI
220 Grapevine flavescence dorée phytoplasma Other Vitis sp. detection Molecular real time PCR yes
Multiplex real-time PCR according to Pelletier et al. (2009)		 no
2014-07-31 EUPH-GRAFDEPI
221 Grapevine flavescence dorée phytoplasma Other Vitis sp. detection Molecular real time PCR yes yes
Triplex real-time (Taq-Man) test based on rpl14 gene sequences (IPADLAB)
2014-07-31 EUPH-GRAFDEPI
222 Grapevine flavescence dorée phytoplasma Other Vitis sp. detection Molecular Conventional PCR yes
Multiplex nested-PCR for simultaneous FD and BN detection (Clair et al. 2003)		 no
2014-07-31 EUPH-GRAFDEPI
223 Grapevine flavescence dorée phytoplasma Other Vitis sp. detection Molecular PCR-RFLP no
no
2014-07-31 EUPH-GRAFDEPI
224 Grapevine flavescence dorée phytoplasma Other Vitis sp. detection Molecular Conventional PCR yes
Direct generic PCR followed by nested group-specific PCR with primers R16(V)F1/R1		 no
2014-07-31 EUPH-GRAFDEPI
225 Grapevine flavescence dorée phytoplasma Other Vitis sp. detection Molecular real time PCR yes
Real-time PCR according to Hren et al. (2007)		 no
2014-07-31 EUPH-GRAFDEPI
226 Begomovirus coheni / Tomato yellow leaf curl virus Leaves Solanum lycopersicum detection Molecular real time PCR, Serological DASI-ELISA yes
TAS-ELISA (based on Thomas
1986)
2018-02-12 ILVODGB
227 'Candidatus Phytoplasma solani', Grapevine flavescence dorée phytoplasma Leaves Vitis sp. detection Molecular real time PCR yes
Multiplex real-time PCR according to Pelletier et al. (2009)		 no
2021-05-18 LNPVAAL
228 Grapevine flavescence dorée phytoplasma Flowers, Fruits, Leaves Vitis vinifera detection Extraction, Molecular Extraction DNA RNA, Molecular LAMP yes
LAMP according to Kogovsek et al. (2015)		 no
2015-11-26 NIB-FITO
229 'Candidatus Phytoplasma solani', Grapevine flavescence dorée phytoplasma Leaves Vitis vinifera detection Molecular Extraction DNA RNA, Molecular real time PCR yes
Real-time PCR according to Hren et al. (2007)		 no
2011-12-30 NIB-FITO
230 Xylella fastidiosa Leaves Citrus x aurantium var. sinensis, Olea europaea, Vitis vinifera detection Molecular Extraction DNA RNA, Molecular real time PCR yes yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)
2015-09-30 LNPVAAL
231 Xylella fastidiosa Leaves Citrus x aurantium var. sinensis, Coffea arabica, Coffea canephora, Prunus persica, Vitis vinifera detection Serological IF

2015-09-30 LNPVAAL
232 Xylella fastidiosa Citrus x aurantium var. sinensis, Coffea arabica, Coffea canephora, Prunus persica, Vitis vinifera detection Molecular Conventional PCR, Molecular Extraction DNA RNA yes yes
Conventional PCR (Minsavage et al.
1994)
2015-09-30 LNPVAAL
233 Pantoea stewartii subsp. stewartii Pure culture Zea mays identification Molecular Conventional PCR yes
PCR (AGES primers)
2011-05-31 EUPH-PANTOEA
234 Pantoea stewartii subsp. stewartii Pure culture Zea mays identification Molecular Conventional PCR yes
PCR (Coplin & Marjercak
2002)
2011-05-31 EUPH-PANTOEA
235 'Candidatus Phytoplasma solani' Leaves, Roots Zea mays detection Molecular Extraction DNA RNA, Molecular real time PCR no
no
2015-06-19 NIB-FITO
236 Pantoea stewartii subsp. stewartii Leaves, Seeds Zea mays detection Molecular Conventional PCR no
no
2017-09-19 IPS2
237 Pantoea stewartii subsp. stewartii Other, Pure culture, Seeds Zea mays detection Molecular real time PCR yes
Real -time PCR (Tambong et al.
2008)		 no
2011-05-31 EUPH-PANTOEA
238 Clavibacter michiganensis Pure culture identification Molecular real time PCR yes
TaqMan real-time PCR test (Oosterhof & Berendsen
2011)		 no
2011-03-28 NIVIP
239 Bursaphelenchus xylophilus Wood detection Molecular real time PCR no
no
2011-02-01 LNPVnema
240 Bursaphelenchus xylophilus Specimen identification Molecular real time PCR no
no
2011-05-01 LNPVnema
241 Bursaphelenchus xylophilus Specimen identification Molecular Conventional PCR no
no
2011-05-01 LNPVnema
242 Globodera pallida, Globodera rostochiensis Specimen identification Molecular Conventional PCR no
no
2010-07-13 LNPVnema
243 Bursaphelenchus xylophilus Wood Pinus sp. detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR no
no
2014-09-01 LNPVnema
244 Meloidogyne enterolobii Specimen identification Molecular Conventional PCR yes
Conventional PCR (Tigano et al. 2010)		 no
2011-11-25 LNPVnema
245 Bursaphelenchus xylophilus Specimen identification Molecular Conventional PCR no
no
2011-05-01 LNPVnema
246 Globodera pallida, Globodera rostochiensis identification Molecular Conventional PCR no
no
2010-07-13 LNPVnema
247 Xanthomonas euvesicatoria pv. euvesicatoria, Xanthomonas euvesicatoria pv. perforans, Xanthomonas hortorum pv. gardneri, Xanthomonas vesicatoria Pure culture identification Molecular Conventional PCR yes
Conventional PCR (according to Koenraadt et al.
2009)
2012-04-17 NIVIP
248 Ditylenchus destructor, Ditylenchus dipsaci Specimen detection and identification Molecular real time PCR yes yes
Real-time PCR tests based on SSU rDNA (provided by ClearDetections)
2013-08-01 CLEAR
249 Heterodera glycines Specimen identification Molecular Conventional PCR no
no
2010-07-07 LNPVnema
250 Heterodera glycines Specimen identification Molecular real time PCR yes
Duplex real-time PCR (Ye
2012)		 no
2015-02-01 LNPVnema
251 Heterodera glycines Specimen identification Molecular PCR-RFLP yes
PCR-ITS-RFLP test (Subbotin et al.
2000)
2010-07-07 LNPVnema
252 Heterodera glycines Specimen identification Molecular Conventional PCR yes
Conventional PCR with species specific primers protocol (Subbotin et al.
2001)		 no
2010-07-07 LNPVnema
253 Clavibacter michiganensis Pure culture identification Serological IF yes
Immunofluorescence test
2011-02-01 NIVIP
254 Bursaphelenchus xylophilus Specimen detection Molecular Conventional PCR yes
Satellite DNA-based PCR technique (Castagnone et al.
2005)		 no
2011-05-01 LNPVnema
255 Bursaphelenchus xylophilus Specimen detection Molecular PCR-RFLP yes
ITS RFLP PCR (Burgermeister et al.
2009)		 no
2011-05-01 LNPVnema
256 Globodera pallida, Globodera rostochiensis Specimen identification Molecular PCR-RFLP yes
ITS PCR-RFLP test (Thiéry & Mugniéry
1996)
2010-07-13 LNPVnema
257 Curtobacterium flaccumfaciens pv. flaccumfaciens identification Molecular Conventional PCR, Molecular Extraction DNA RNA yes yes
Conventional PCR (Tegli et al.
2002)
2011-06-24 NIVIP
258 Globodera pallida, Globodera rostochiensis Specimen detection and identification Molecular real time RT PCR yes
RNA-specific real-time PCR (Beniers et al.
2014)		 no
2014-07-01 NAK
259 Xylella fastidiosa Specimen detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR yes yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no
2016-05-31 LNPVAAL
260 Meloidogyne chitwoodi, Meloidogyne fallax Specimen detection Molecular PCR-RFLP no
no
2010-09-01 LNPVnema
261 Bursaphelenchus xylophilus Specimen identification Molecular Conventional PCR no
no
2011-05-01 LNPVnema
264 Globodera pallida, Globodera rostochiensis Specimen identification Molecular real time PCR no
no
2013-08-16 EVIRAPQL
265 Meloidogyne chitwoodi, Meloidogyne fallax Specimen identification Molecular Conventional PCR yes
PCR Zijlstra et al. 2000		 no
2010-01-01 LNPVnema
266 Bursaphelenchus xylophilus Wood detection Molecular real time PCR no
no
2011-02-01 LNPVnema
267 Globodera pallida, Globodera rostochiensis Specimen identification Molecular Conventional PCR yes
Multiplex PCR test (Bulman & Marshall
1997)
2010-07-13 LNPVnema
268 Meloidogyne chitwoodi, Meloidogyne fallax Specimen detection Molecular Conventional PCR yes
PCR Wishart et al. 2002
2010-09-01 LNPVnema
269 Meloidogyne chitwoodi Specimen identification Molecular Conventional PCR yes
PCR Wishart et al. 2002
2012-01-01 ILVODGB
270 Liriomyza huidobrensis Specimen identification Extraction, Morphological yes
Morphological identification
2009-04-14 LNPHL
279 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR targeting 16S rRNA gene (according to Li et al.
2006)		 no
2020-07-10 LNPVRAPT
280 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR targeting 16S rRNA gene (according to Li et al.
2006)		 no
2020-07-10 LNPVRAPT
281 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR targeting hyvI/hyvII gene (according to Morgan et al. 2012)		 no
2020-07-10 LNPVRAPT
282 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR targeting hyvI/hyvII gene (according to Morgan et al. 2012)		 no
2020-07-10 LNPVRAPT
283 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR targeting hyvI/hyvII gene (according to Morgan et al. 2012)		 no
2020-07-10 LNPVRAPT
284 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR targeting hyvI/hyvII gene (according to Morgan et al. 2012)		 no
2020-07-10 LNPVRAPT
285 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR targeting 16S rRNA gene (according to Bertolini et al. (2010
2014))		 no
2020-07-10 LNPVRAPT
286 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR targeting 16S rRNA gene (according to Bertolini et al. (2010
2014))		 no
2020-07-10 LNPVRAPT
287 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Extraction DNA RNA, Molecular real time PCR yes no
Real-time PCR targeting 16S rRNA gene (according to Bertolini et al. (2010
2014))		 no
2020-07-10 LNPVRAPT
288 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Extraction DNA RNA, Molecular real time PCR yes no
Real-time PCR targeting 16S rRNA gene (according to Bertolini et al. (2010
2014))		 no
2020-07-10 LNPVRAPT
289 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Conventional PCR no no
Conventional PCR targeting rplKAJL – rpoBC operon gene (according to Hocquellet et al.
1999)		 no
2020-07-10 LNPVRAPT
290 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter asiaticus' Leaves Citrus sp. detection and identification Molecular Conventional PCR no no
Conventional PCR targeting rplKAJL – rpoBC operon gene (according to Hocquellet et al.
1999)		 no
2020-07-10 LNPVRAPT
292 Bursaphelenchus xylophilus Other identification Molecular real time PCR yes no
no
2020-07-30 CLEAR
296 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Molecular Conventional RT PCR, Molecular Extraction DNA RNA no, yes no, yes
no, yes
Levy
L. & Hadidi
A. 1994. A simple and rapid method for processing tissue infected with Plum pox potyvirus for use with specific 3′ non-coding region RT-PCR assays. EPPO Bulletin
24: 595– 604.		 2020-06-30 NIVIP
297 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Molecular Conventional RT PCR, Molecular Extraction DNA RNA no, yes yes
no, yes
Wetzel T
Candresse T
Ravelonandro M & Dunez J (1991) A polymerase chain reaction assay adapted to Plum pox potyvirus detection. Journal of Virological Methods 33
355-365		 2020-06-30 NIVIP
298 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no, yes
no
2020-06-30 NIVIP
299 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no, yes
no, yes
Schneider WL
Sherman DJ
Stone AL
Damsteegt VD & Fredericket RD (2004) Specific detection and quantification of Plum pox virus by real-time fluorescent reverse transcription PCR. Journal of Virological Methods
120
97–105		 2020-06-30 NIVIP
300 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no, yes
no
2020-06-30 NIVIP
301 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Serological DAS-ELISA yes yes
no
2020-06-30 NIVIP
302 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Serological DAS-ELISA yes no
no
2020-06-30 NIVIP
303 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Serological DAS-ELISA yes no
no
2020-06-30 NIVIP
304 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Molecular Conventional RT PCR, Molecular Extraction DNA RNA no, yes no, yes
no, yes
Levy
L. & Hadidi
A. 1994. A simple and rapid method for processing tissue infected with Plum pox potyvirus for use with specific 3′ non-coding region RT-PCR assays. EPPO Bulletin
24: 595– 604.		 2020-06-30 NIVIP
305 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Molecular Conventional RT PCR, Molecular Extraction DNA RNA no, yes yes
no, yes
Wetzel T
Candresse T
Ravelonandro M & Dunez J (1991) A polymerase chain reaction assay adapted to Plum pox potyvirus detection. Journal of Virological Methods 33
355-365		 2020-06-30 NIVIP
306 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Molecular Extraction DNA RNA, Molecular real time RT PCR yes no, yes
no
2020-06-30 NIVIP
307 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no, yes
no, yes
Schneider WL
Sherman DJ
Stone AL
Damsteegt VD & Fredericket RD (2004) Specific detection and quantification of Plum pox virus by real-time fluorescent reverse transcription PCR. Journal of Virological Methods
120
97–105		 2020-06-30 NIVIP
308 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no, yes
no
2020-06-30 NIVIP
309 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Serological DAS-ELISA yes yes
no
2020-06-30 NIVIP
310 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Serological DAS-ELISA yes no
no
2020-06-30 NIVIP
311 Potyvirus plumpoxi / Plum pox virus Leaves Nicotiana benthamiana, Prunus, Prunus avium, Prunus domestica, Prunus persica detection Serological DAS-ELISA yes no
no
2020-06-30 NIVIP
319 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Serological DAS-ELISA yes yes
DAS ELISA Generic or Specific antisera (Appendix 2)
2020-12-14 NIB-FITO
320 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Serological DAS-ELISA yes yes
DAS ELISA Generic or Specific antisera (Appendix 2)
2020-12-14 NIB-FITO
321 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Serological Lateral Flow Device yes yes
Lateral flow devices		 no
2020-12-14 NIB-FITO
322 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Serological Lateral Flow Device yes yes
Lateral flow devices		 no
2020-12-14 NIB-FITO
323 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Molecular Conventional RT PCR, Molecular Extraction DNA RNA no, yes no, yes
no
2020-12-14 NIB-FITO
324 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR yes yes
Real-time RT-PCR for TSWV (Boonham et al.
2002)		 no
2020-12-14 NIB-FITO
325 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no, yes
no
2020-12-14 NIB-FITO
326 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no, yes
no
2020-12-14 NIB-FITO
327 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Serological DAS-ELISA yes yes
DAS ELISA Generic or Specific antisera (Appendix 2)
2020-12-14 NIB-FITO
328 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Serological DAS-ELISA yes yes
DAS ELISA Generic or Specific antisera (Appendix 2)
2020-12-14 NIB-FITO
329 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Serological Lateral Flow Device yes yes
Lateral flow devices		 no
2020-12-14 NIB-FITO
330 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Serological Lateral Flow Device yes yes
Lateral flow devices		 no
2020-12-14 NIB-FITO
331 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Molecular Conventional RT PCR, Molecular Extraction DNA RNA no, yes no, yes
no
2020-12-14 NIB-FITO
332 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes yes
Real-time RT-PCR for TSWV (Boonham et al.
2002)		 no
2020-12-14 NIB-FITO
333 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no, yes
no
2020-12-14 NIB-FITO
334 Orthotospovirus tomatomaculae / Tomato spotted wilt virus Leaves Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no, yes
no
2020-12-14 NIB-FITO
335 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter americanus', 'Candidatus Liberibacter asiaticus' Other Citrus reticulata, Citrus x aurantium var. sinensis detection Molecular Conventional PCR, Molecular real time PCR

2021-03-08 IVIA-B
336 Erwinia amylovora Flowers, Leaves, Wood, Woody cuttings detection Molecular LAMP, Molecular real time PCR

2021-03-08 IVIA-B
337 Clavibacter sepedonicus, Ralstonia pseudosolanacearum, Ralstonia solanacearum, Ralstonia syzygii Other, Tubers Solanum tuberosum detection Isolation, Molecular Conventional PCR, Molecular real time PCR

2021-03-08 IVIA-B
339 Xylella fastidiosa Other Olea europaea detection Molecular Conventional PCR, Molecular Extraction DNA RNA, Molecular real time PCR

2021-03-08 IVIA-B
340 Xylella fastidiosa Leaves Coffea sp., Lavandula angustifolia, Nerium oleander, Olea europaea, Polygala myrtifolia, Prunus avium, Vitis vinifera detection Molecular real time PCR

2021-03-08 IVIA-B
341 Xylella fastidiosa Other Polygala myrtifolia detection Molecular Extraction DNA RNA, Molecular real time PCR

2021-03-08 IVIA-B
342 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Fruits, Leaves Capsicum annuum, Solanum lycopersicum detection Molecular Conventional RT PCR, Molecular Extraction DNA RNA no, yes no, yes
no
2020-12-29 CREA-DC-Roma
343 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Fruits, Leaves Capsicum annuum, Solanum lycopersicum detection Molecular Conventional RT PCR, Molecular Extraction DNA RNA yes no
no
2020-12-29 CREA-DC-Roma
344 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Fruits, Leaves Capsicum annuum, Solanum lycopersicum detection Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no
no
2020-12-29 CREA-DC-Roma
345 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Fruits, Leaves Capsicum annuum, Solanum lycopersicum detection Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no
no
2020-12-29 CREA-DC-Roma
346 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Fruits, Leaves Capsicum annuum, Solanum lycopersicum detection Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no
no
2020-12-29 CREA-DC-Roma
350 Ditylenchus dipsaci Seeds Medicago sativa detection and identification Molecular real time RT PCR, Morphological

2019-03-08 GEVES
351 Ditylenchus dipsaci, Ditylenchus gigas Seeds Medicago sativa, Vicia faba subsp. minor detection and identification Molecular Conventional PCR, Morphological

2015-03-22 GEVES
353 Ralstonia pseudosolanacearum, Ralstonia solanacearum Herbaceous cuttings, Shoots, Woody cuttings Anthurium, Begonia, Capsicum annuum, Pelargonium, Rosa, Solanum lycopersicum, Solanum melongena detection Molecular real time PCR yes yes
Real-time TaqMan PCR test (Weller et al.
2000)		 no
2019-09-12 NIVIP
354 Xanthomonas citri pv. citri Fruits, Leaves Citrus x aurantium var. sinensis, Citrus x limon detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
no, yes
Cubero and Graham
2005		 2021-05-25 CREA-DC-Roma
361 Xanthomonas citri pv. citri Fruits, Leaves citrus (general) detection Molecular Conventional PCR no no
no
2021-08-05 LNPVRAPT
362 Xanthomonas citri pv. citri Fruits, Leaves citrus (general) detection Molecular Conventional PCR no no
no
2021-08-05 LNPVRAPT
363 Xanthomonas citri pv. citri Fruits, Leaves citrus (general) detection Molecular Conventional PCR no no
no
2021-08-05 LNPVRAPT
364 Xanthomonas citri pv. citri Fruits, Leaves citrus (general) detection Molecular Conventional PCR no no
no
2021-08-05 LNPVRAPT
365 Xanthomonas citri pv. citri Fruits, Leaves citrus (general) detection Molecular Conventional PCR no no
no
2021-08-05 LNPVRAPT
366 Xanthomonas citri pv. citri Fruits, Leaves citrus (general) detection Molecular Conventional PCR yes no
no
2021-08-05 LNPVRAPT
367 Xanthomonas citri pv. citri Fruits, Leaves citrus (general) detection Molecular real time PCR no no
no
2021-08-05 LNPVRAPT
368 Xanthomonas citri pv. citri Fruits, Leaves citrus (general) detection Molecular real time PCR no no
no
2021-08-05 LNPVRAPT
369 Xanthomonas citri pv. citri Fruits, Leaves citrus (general) detection Molecular real time PCR no no
no
2021-08-05 LNPVRAPT
370 Xanthomonas citri pv. citri Fruits, Leaves citrus (general) detection Molecular LAMP no no
no
2021-08-05 LNPVRAPT
371 Xylella fastidiosa Shoots Helichrysum italicum detection Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 yes
QuickPick SML Plant DNA kit (Bio-Nobile)
Real-time PCR -simplex (Harper et al.
2010;errtum 2013)		 2020-12-24 LNPVAAL
372 Xylella fastidiosa Specimen Philaenus spumarius detection Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR - duplex (Harper et al.
2010; erratum 2013)		 no
2020-03-04 LNPVAAL
374 Xylella fastidiosa Leaves, Shoots Helichrysum italicum, Lavandula sp., Polygala myrtifolia detection Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR (adapted from Ouyang et al.
2013)		 no, yes
QuickPick SML Plant DNA kit (Bio-Nobile)		 2021-09-16 LNPVAAL
375 Xylella fastidiosa Herbaceous cuttings, Leaves, Shoots, Woody cuttings Citrus x aurantium var. sinensis, Olea europaea, Quercus ilex, Vitis vinifera detection Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 yes
QuickPick SML Plant DNA kit (Bio-Nobile)
Real-time PCR -simplex (Harper et al.
2010;errtum 2013)		 2020-03-25 LNPVAAL
385 Xylella fastidiosa Other Coffea arabica, Lavandula stoechas, Nerium oleander, Olea europaea, Polygala myrtifolia, Prunus avium detection and identification Molecular real time PCR no yes
Real-time PCR (adapted from Ouyang et al.
2013)		 no
2019-09-02 NIVIP
386 Bursaphelenchus xylophilus Woody cuttings Pinus detection Extraction yes
Mistifier technique (from plant material and vectors)		 no
2020-09-25 BBBROE
387 Xylella fastidiosa Leaves, Shoots identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
no, yes
QuickPick SML Plant DNA kit (Bio-Nobile)		 2021-12-27 LNPVAAL
388 Xylella fastidiosa Leaves, Shoots, Woody cuttings Olea europaea detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no
2019-10-28 ISPP-CNR
389 Xylella fastidiosa Leaves Polygala myrtifolia detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no, yes
2021-01-30 ISPP-CNR
390 Xylella fastidiosa Other detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no, yes
2021-01-30 ISPP-CNR
391 Xylella fastidiosa Wood Prunus avium detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no
2019-10-28 ISPP-CNR
392 Xylella fastidiosa Shoots Lavandula detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no
2019-10-28 ISPP-CNR
393 Xylella fastidiosa Leaves Nerium oleander detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no
2019-01-30 ISPP-CNR
394 Xylella fastidiosa Shoots Salvia rosmarinus detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no
2019-10-28 ISPP-CNR
395 Xylella fastidiosa Leaves Citrus x aurantium var. sinensis detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no
2019-10-28 ISPP-CNR
396 Xylella fastidiosa Leaves Vitis vinifera detection Extraction, Molecular Extraction DNA RNA, Molecular real time PCR no, yes no, yes
Real-time PCR - simplex (Harper et al.
2010; erratum 2013)		 no
2019-01-30 ISPP-CNR
397 Xylella fastidiosa Leaves, Shoots Cistus monspeliensis, Citrus maxima, Citrus reticulata, Citrus x aurantium var. clementina, Citrus x aurantium var. sinensis, Citrus x limon, Helichrysum italicum, Lavandula, Nerium oleander, Olea europaea, Polygala myrtifolia, Prunus cerasus, Prunus dulcis, Quercus ilex, Quercus sp., Vitis vinifera detection Molecular other yes no
no
2021-12-16 INRA-ANGERS
398 Xylella fastidiosa Leaves, Other, Shoots Arbutus, Asparagus officinalis, Centranthus, Cistus, Cupressus, Ficus, Genista, Helichrysum petiolare, Juglans, Lavandula, Myrtus, Nerium, Olea, Phagnalon, Platanus, Polygala myrtifolia, Prunus, Quercus, Spartium detection and identification Molecular Conventional PCR no no
no
2021-12-16 INRA-ANGERS
399 Globodera pallida, Globodera rostochiensis Other detection and identification Molecular real time PCR no no
no
2021-10-21 LNPVnema
400 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Seeds Capsicum, Solanum lycopersicum detection Extraction, Molecular Extraction DNA RNA, Molecular real time RT PCR yes yes
Real-time RT-PCR Menzel and Winter (2021)		 no
2021-12-01 NIVIP
401 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Seeds Capsicum, Solanum lycopersicum detection Extraction, Molecular Extraction DNA RNA, Molecular real time RT PCR yes yes
Real-time RT-PCR ISF (2020) - CaTa28 and CSP1325 primers and probes		 no
2021-12-01 NIVIP
402 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Seeds Capsicum, Solanum lycopersicum detection Extraction, Molecular Extraction DNA RNA, Molecular real time RT PCR no, yes no, yes
no
2021-12-01 NIVIP
403 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Seeds Capsicum, Solanum lycopersicum detection Extraction, Molecular Conventional RT PCR, Molecular Extraction DNA RNA yes yes
One step conventional RT-PCR Loewe (Rodríguez-Mendoza et al. 2019)		 no
2021-12-01 NIVIP
404 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Seeds Capsicum, Solanum lycopersicum detection Extraction, Molecular Conventional RT PCR, Molecular Extraction DNA RNA no, yes yes
One step conventional RT-PCR Alkowni et al. (2019)		 no
2021-12-01 NIVIP
405 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Seeds Capsicum, Solanum lycopersicum detection Extraction, Molecular Extraction DNA RNA, Molecular LAMP yes no, yes
no
2021-12-01 NIVIP
406 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Seeds Capsicum, Solanum lycopersicum detection Extraction, Molecular Extraction DNA RNA, Molecular LAMP yes no, yes
no
2021-12-01 NIVIP
407 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Leaves, Seeds Solanum lycopersicum detection Extraction, Serological DAS-ELISA yes no, yes
no
2021-12-01 NIVIP
408 Xanthomonas euvesicatoria pv. euvesicatoria, Xanthomonas euvesicatoria pv. perforans, Xanthomonas hortorum pv. gardneri, Xanthomonas vesicatoria Seeds Solanum lycopersicum detection Molecular Conventional PCR no yes
Conventional PCR (according to Koenraadt et al.
2009)		 no
2022-01-22 CREA-DC-Roma
409 Bactrocera dorsalis Specimen identification Molecular Extraction DNA RNA, Morphological

2021-10-15 LNPVENTOMO
410 Xanthomonas euvesicatoria pv. euvesicatoria, Xanthomonas euvesicatoria pv. perforans, Xanthomonas hortorum pv. gardneri, Xanthomonas vesicatoria Seeds Solanum lycopersicum detection Molecular real time RT PCR no

2022-02-10 CREA-DC-Roma
411 Pantoea stewartii subsp. indologenes, Pantoea stewartii subsp. stewartii Other detection and identification Molecular real time RT PCR

2021-10-01 IVIA-B
413 'Candidatus Liberibacter africanus', 'Candidatus Liberibacter americanus', 'Candidatus Liberibacter asiaticus' Other detection Molecular Conventional PCR, Molecular Conventional RT PCR

2021-09-20 IVIA-B
414 Xanthomonas citri pv. citri Other Citrus detection and identification Molecular Conventional PCR, Molecular real time PCR

2020-01-23 IVIA-B
415 Xylella fastidiosa Other Olea europaea, Prunus avium, Salvia rosmarinus, Vitis vinifera detection Extraction, Molecular Conventional PCR, Molecular real time PCR

2022-02-18 IVIA-B
416 Xylella fastidiosa Other Prunus dulcis detection Extraction, Molecular Conventional RT PCR

2022-02-18 IVIA-B
417 Xanthomonas citri pv. citri Other detection and identification Molecular real time PCR

2022-02-18 IVIA-B
419 'Candidatus Liberibacter solanacearum' Other Daucus carota subsp. sativus, Solanum tuberosum detection Extraction, Molecular Conventional RT PCR

2022-02-18 IVIA-B
420 Clavibacter sepedonicus Tubers Solanum tuberosum detection Molecular Conventional PCR yes
PCR (Pastrik
2000)
2018-04-16 IPS2
425 Potyvirus plumpoxi / Plum pox virus Leaves Prunus sp. detection Serological DAS-ELISA

2018-05-21 SPAOLO
433 Xanthomonas axonopodis pv. dieffenbachiae Pure culture identification Serological IF no yes
Immunofluorescence test		 no
2017-10-18 NIVIP
434 Xanthomonas fragariae Pure culture identification Serological IF no yes
IF - EU (1998)		 no
2017-10-18 NIVIP
435 Begomovirus solanumdelhiense / Tomato leaf curl New Delhi virus Leaves Cucurbitaceae detection Serological DAS-ELISA yes no
no
2022-05-05 CREA-DC-Roma
436 Begomovirus solanumdelhiense / Tomato leaf curl New Delhi virus Leaves Cucurbitaceae detection Serological DAS-ELISA yes no
no
2022-05-05 CREA-DC-Roma
437 Begomovirus solanumdelhiense / Tomato leaf curl New Delhi virus Leaves Cucurbitaceae detection Molecular LAMP no no
no
2022-05-05 CREA-DC-Roma
438 Begomovirus solanumdelhiense / Tomato leaf curl New Delhi virus Leaves Cucurbitaceae detection Molecular real time PCR no no
no
2022-05-05 CREA-DC-Roma
439 Begomovirus solanumdelhiense / Tomato leaf curl New Delhi virus Leaves Cucurbitaceae detection and identification Molecular real time PCR no no
no
2022-05-05 CREA-DC-Roma
440 Nanovirus necropisi Leaves Lupinus, Pisum sativum, Vicia faba detection and identification Molecular Conventional PCR, Molecular Extraction DNA RNA no no
no
2021-03-02 JKI-EP
441 Begomovirus Leaves detection Molecular Conventional PCR no no
no
2016-03-16 LNPVAAL
442 Begomovirus Leaves detection Molecular Conventional PCR no no
no
2017-05-01 LNPVAAL
443 Begomovirus Leaves detection Molecular Conventional PCR no no
no
2017-05-01 LNPVAAL
444 Begomovirus Leaves detection Molecular Conventional PCR no no
no
2017-05-01 LNPVAAL
445 Xanthomonas citri pv. aurantifolii, Xanthomonas citri pv. citri Fruits, Leaves Citrus x aurantiifolia, Citrus x aurantium var. sinensis, Citrus x limon detection Molecular Conventional PCR, Molecular real time PCR no no
no, yes
Cubero and Graham
2005		 2022-09-28 CREA-DC-Roma
448 Ralstonia solanacearum species complex Pure culture detection and identification Molecular Conventional PCR, Molecular real time PCR, Other yes
no
2023-01-06 IVIA-B
449 Xylella fastidiosa Leaves, Pure culture Calicotome, Catharanthus roseus, Coffea arabica, Genista, Helichrysum, Laurus nobilis, Myrtus communis, Olea europaea, Polygala, Salvia rosmarinus identification Molecular real time PCR no no
no
2018-08-14 Fera
450 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Fruits, Leaves Capsicum sp., Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR yes yes
Real-time RT-PCR Menzel and Winter (2021)
2023-02-06 NIVIP
451 Xylella fastidiosa Leaves, Woody cuttings Olea europaea, Quercus ilex detection Molecular Extraction DNA RNA, Molecular real time PCR yes no, yes
Real-time PCR - duplex (Harper et al.
2010; erratum 2013)		 no, yes
Real-time PCR Harper et al.
2010		 2023-02-01 LNPVAAL
456 Synchytrium endobioticum Soil, Tubers Solanum tuberosum detection and identification Molecular real time PCR no yes
Taqman Real-time PCR (based on Smith et al.
2014)		 no
2021-12-13 NIVIP
457 Synchytrium endobioticum Soil, Tubers Solanum tuberosum detection and identification Molecular real time PCR no yes
Real-time PCR (Van Gent-Pelzer et al.
2010)		 no
2021-12-13 NIVIP
459 Elsinoë Fruits Citrus detection Molecular real time PCR no no
no
2023-05-10 NIVIP
462 Xanthomonas vesicatoria Herbaceous cuttings Capsicum annuum, Solanum lycopersicum detection Isolation

2015-06-12 NIVIP
464 Ipomovirus lycopersici / Tomato mild mottle virus Leaves Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular HTS yes no
no
2023-05-29 NIB-FITO
465 Phytophthora ramorum Leaves Camellia, Fagus, Kalmia, Pieris, Rhododendron ponticum, Vaccinium, Veronica, Viburnum detection Molecular real time PCR yes no
no
2023-02-27 NAKTUINBOUW
466 Bretziella fagacearum Pure culture detection and identification Morphological yes
Morphological identification in culture		 no
2022-08-22 NIVIP
467 Davidsoniella virescens Pure culture detection and identification Morphological no
no
2022-10-22 NIVIP
468 Xylella fastidiosa Leaves, Shoots, Water Citrus, Lavandula angustifolia, Olea europaea, Prunus identification Molecular real time PCR no yes
Tetraplex real-time PCR (Dupas et al.
2019)		 no
2023-09-22 NIVIP
469 Xylella fastidiosa Leaves, Shoots, Water Citrus, Lavandula angustifolia, Olea europaea, Prunus identification Molecular real time PCR no yes
Real-time PCR test (Hodgetts et al.
2021)		 no
2023-09-22 NIVIP
470 Geosmithia morbida Pure culture detection and identification Morphological no
no
2021-11-30 NIVIP
471 Fusarium circinatum Pure culture detection and identification Morphological yes
Morphological identification in pure culture on PDA and SNA		 no
2020-01-14 NIVIP
473 Phytophthora kernoviae Pure culture identification Morphological yes
Morphological identification P5ARP [H]		 no
2023-03-30 NIVIP
474 Phytophthora lateralis Pure culture identification Morphological yes
Morphological identification on V8 agar (ANSES recipe)		 no
2023-04-12 NIVIP
475 Phytophthora ramorum Pure culture identification Morphological yes
no
2023-04-13 NIVIP
476 Pantoea stewartii subsp. stewartii Shoots Zea mays detection Isolation yes
Isolation on King’s B agar		 no
2022-04-14 NIVIP
477 Pantoea stewartii subsp. stewartii Pure culture Zea mays identification Pathogenicity yes
Pathogenecity test		 no
2022-04-06 NIVIP
478 Pantoea stewartii subsp. stewartii Pure culture identification MALDI-TOF no
no
2022-04-25 NIVIP
479 Pantoea stewartii subsp. stewartii Pure culture, Shoots Zea mays detection and identification Molecular real time RT PCR no yes
no
2022-04-28 NIVIP
480 Meloidogyne graminicola Specimen identification Molecular Conventional PCR, Molecular Extraction DNA RNA no no
no
2023-10-31 ILVODGB
481 Meloidogyne graminicola Specimen identification Molecular Conventional PCR, Molecular Extraction DNA RNA no, yes no
no
2023-07-31 ILVODGB
482 Meloidogyne graminicola Specimen identification Molecular Conventional PCR, Molecular Extraction DNA RNA no no
no
2023-10-31 ILVODGB
483 Meloidogyne graminicola Specimen identification Molecular Conventional PCR, Molecular Extraction DNA RNA no, yes no
no
2023-07-31 ILVODGB
485 Meloidogyne graminicola Specimen identification Molecular Conventional PCR, Molecular Extraction DNA RNA no no
no
2023-10-31 ILVODGB
486 Meloidogyne graminicola Specimen identification Molecular Extraction DNA RNA, Molecular real time PCR no no
no
2023-10-31 ILVODGB
487 Meloidogyne graminicola Specimen identification Molecular Conventional PCR, Molecular Extraction DNA RNA no no
no
2023-10-31 ILVODGB
488 Meloidogyne graminicola Specimen identification Molecular Conventional PCR, Molecular Extraction DNA RNA no no
no
2023-10-31 ILVODGB
490 Begomovirus Leaves Cucurbitaceae, Solanum lycopersicum detection Molecular Conventional PCR, Molecular Extraction DNA RNA yes yes
Conventional PCR Li et al. (2004)
Conventional PCR Saison and Gentit (2015)
Conventional PCR Wyatt and Brown (1996)		 no
2023-09-29 NIB-FITO
491 Emaravirus rosae Leaves Rosa detection Extraction, Molecular real time RT PCR yes no
no
2023-08-21 NIVIP
495 Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola Pure culture identification MALDI-TOF no
no
2023-09-27 NIVIP
496 Tobamovirus fructirugosum / Tomato brown rugose fruit virus Leaves, Seeds Capsicum annuum, Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR yes yes
Real-time RT-PCR Bernabé-Orts et al. (2021)		 no
2023-12-14 NIB-FITO
498 Xylella fastidiosa Other Citrus, Lavandula, Oleaceae, Prunus dulcis detection and identification Molecular real time PCR

2024-01-24 IVIA-B
499 Xylella fastidiosa Other Citrus, Lavandula, Olea, Prunus detection and identification Molecular real time PCR

2024-01-24 IVIA-B
500 Xylella fastidiosa Woody cuttings Prunus domestica, Prunus dulcis, Vitis vinifera detection and identification Molecular real time PCR

2024-01-24 IVIA-B
501 Curtobacterium flaccumfaciens pv. flaccumfaciens Herbaceous cuttings Phaseolus vulgaris detection Isolation, Molecular Conventional PCR, Molecular Extraction DNA RNA, Molecular real time PCR, Pathogenicity

2024-01-24 IVIA-B
502 Xylella fastidiosa Other Citrus detection Molecular Conventional PCR, Molecular real time PCR

2024-01-24 IVIA-B
503 'Candidatus Liberibacter asiaticus' Other Citrus detection Molecular real time PCR

2024-01-24 IVIA-B
504 Liberibacter, Xanthomonas citri, Xylella fastidiosa Other Citrus detection Molecular Conventional PCR, Molecular real time PCR

2024-01-24 IVIA-B
505 Potexvirus pepini / Pepino mosaic virus Leaves Nicotiana occidentalis, Solanum lycopersicum detection and identification Serological DAS-ELISA no yes
DAS ELISA		 no
2015-08-24 NIVIP
506 Pantoea stewartii subsp. stewartii Seeds Zea mays detection Molecular real time PCR no no

2024-03-01 CREA-DC-Roma
507 Pantoea stewartii subsp. stewartii Seeds Zea mays detection Molecular real time PCR no no

2024-03-01 CREA-DC-Roma
508 Pantoea stewartii subsp. stewartii Seeds detection Molecular real time PCR no yes
Real -time PCR (Tambong et al.
2008)
2024-03-01 CREA-DC-Roma
509 Xylella fastidiosa Leaves identification Molecular real time PCR

2024-03-11 CREA-DC-Roma
510 Cryphonectria parasitica Pure culture Castanea sativa detection and identification Molecular real time PCR no no
no
2021-04-30 AGRIUNITO
511 Cryphonectria parasitica Pure culture Castanea sativa detection and identification Molecular real time PCR no no
no
2021-04-30 AGRIUNITO
512 Viruses and viroids Flowers, Fruits, Leaves Buddleia davidii, Capsicum, Citrus, Cucumis sativus, Fragaria, Malva, Nicotiana benthamiana, Rosa, Rubus idaeus, Salvia, Sambucus nigra, Solanum lycopersicum, Vicia faba detection and identification Molecular HTS no
no
2020-07-13 NIVIP
513 Orthotospovirus chrysanthinecrocaulis / Chrysanthemum stem necrosis virus Leaves Chrysanthemum x morifolium, Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR yes yes
Real-time RT-PCR test for CSNV (Boben et al.
2007)		 no
2024-04-30 NIB-FITO
514 Orthotospovirus, Orthotospovirus chrysanthinecrocaulis / Chrysanthemum stem necrosis virus Leaves Chrysanthemum x morifolium, Solanum lycopersicum detection Molecular Conventional RT PCR, Molecular Extraction DNA RNA yes yes
Conventional RT-PCR (Hassani-Mehraban et al.
2016)		 no
2024-04-30 NIB-FITO
516 Meloidogyne graminicola Roots Oryza sativa detection Extraction, Molecular Conventional PCR, Molecular Extraction DNA RNA no no
no
2017-03-09 ISPP-CNR
518 Phytophthora fragariae Roots detection Molecular real time PCR yes no
no
2018-09-01 NAKTUINBOUW
519 Phytophthora cinnamomi Roots, Soil, Wood Castanea sativa, Juglans regia detection and identification Molecular real time PCR no no
no
2024-07-05 CREA-DC-Roma
520 Meloidogyne graminicola Specimen identification Molecular Extraction DNA RNA, Molecular real time PCR no no
no
2024-08-21 LNPVnema
521 Meloidogyne graminicola Specimen identification Molecular real time PCR no no
no
2024-08-21 LNPVnema
522 Meloidogyne enterolobii Specimen identification Molecular Extraction DNA RNA, Molecular real time PCR no no, yes
no
2024-08-21 LNPVnema
523 Curtobacterium flaccumfaciens pv. flaccumfaciens Pure culture Phaseolus vulgaris identification Pathogenicity yes
Pathogenicity test method C		 no
2022-04-22 NIVIP
524 Phytoplasma Leaves Catharanthus roseus, Fragaria x ananassa, Malus domestica, Morus, Prunus cerasus, Prunus domestica, Prunus persica, Ribes rubrum, Ulmus, Vitis vinifera detection and identification Molecular Conventional PCR no no
no
2015-02-27 CRAW
525 Xylella fastidiosa Woody cuttings Prunus domestica, Prunus dulcis, Vitis vinifera detection Molecular Extraction DNA RNA, Molecular real time PCR no yes
Real-time PCR (Harper et al.
2010; erratum 2013)		 no
2024-09-13 LNPVAAL
526 Xylella fastidiosa Woody cuttings Prunus domestica, Prunus dulcis, Vitis vinifera detection Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR (Harper et al.
2010; erratum 2013)		 no
2024-09-13 LNPVAAL
527 Xylella fastidiosa Woody cuttings Prunus domestica, Prunus dulcis, Vitis vinifera detection Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR (Harper et al.
2010; erratum 2013)		 no
2024-09-13 LNPVAAL
528 Xylella fastidiosa Woody cuttings Prunus domestica, Prunus dulcis, Vitis vinifera detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Tetraplex real-time PCR (Dupas et al.
2019)		 no
2024-09-13 LNPVAAL
529 Xylella fastidiosa Woody cuttings Prunus domestica, Prunus dulcis, Vitis vinifera detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no yes
Real-time PCR test (Hodgetts et al.
2021)		 no
2024-09-18 LNPVAAL
530 Xylella fastidiosa Woody cuttings Prunus domestica, Prunus dulcis, Vitis vinifera detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no yes
Real-time PCR test (Hodgetts et al.
2021)		 no
2024-09-18 LNPVAAL
531 Xylella fastidiosa Woody cuttings Prunus domestica, Prunus dulcis, Vitis vinifera detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR test (Hodgetts et al.
2021)		 no
2024-09-20 LNPVAAL
532 Xylella fastidiosa Woody cuttings Prunus domestica, Prunus dulcis, Vitis vinifera detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no yes
Real-time PCR test (Hodgetts et al.
2021)		 no
2024-09-20 LNPVAAL
533 Xylella fastidiosa Woody cuttings Prunus domestica, Prunus dulcis, Vitis vinifera detection and identification Molecular Extraction DNA RNA, Molecular real time PCR no, yes yes
Real-time PCR test (Hodgetts et al.
2021)		 no
2024-09-20 LNPVAAL
534 Phytoplasma Other detection Molecular Conventional PCR, Molecular Extraction DNA RNA no, yes no, yes
Conventional nested PCR using the primers P1/P7 and R16F2n/R16R2		 no
2024-08-22 NIB-FITO
535 Phytoplasma Other detection and identification Molecular Extraction DNA RNA, Molecular real time PCR yes no, yes
no
2024-08-22 NIB-FITO
537 Tobamovirus maculatessellati / Tomato mottle mosaic virus Other detection and identification Molecular Conventional RT PCR yes no
no
2024-09-17 NIB-FITO
538 Tobamovirus maculatessellati / Tomato mottle mosaic virus Other detection and identification Molecular Conventional RT PCR yes no
no
2024-09-17 NIB-FITO
539 Tobamovirus maculatessellati / Tomato mottle mosaic virus Other detection and identification Molecular Conventional RT PCR yes no
no
2024-09-17 NIB-FITO
540 Tobamovirus maculatessellati / Tomato mottle mosaic virus Other detection and identification Molecular real time RT PCR yes no
no
2024-09-17 NIB-FITO
541 Tobamovirus maculatessellati / Tomato mottle mosaic virus Other detection and identification Molecular real time RT PCR yes no
no
2024-09-17 NIB-FITO
542 Tobamovirus maculatessellati / Tomato mottle mosaic virus Other detection and identification Molecular real time RT PCR yes no
no
2024-09-17 NIB-FITO
543 Tobamovirus maculatessellati / Tomato mottle mosaic virus Other detection and identification Molecular real time RT PCR yes no
no
2024-09-17 NIB-FITO
544 Tobamovirus maculatessellati / Tomato mottle mosaic virus Other detection and identification Molecular real time RT PCR yes no
no
2024-09-17 NIB-FITO
545 Tobamovirus maculatessellati / Tomato mottle mosaic virus Other detection and identification Molecular other yes no
no
2024-09-17 NIB-FITO
546 Tobamovirus maculatessellati / Tomato mottle mosaic virus Other detection and identification Molecular LAMP yes no
no
2024-09-17 NIB-FITO
547 Bemisia tabaci Specimen identification Molecular Extraction DNA RNA, Molecular LAMP no, yes no
no
2024-03-08 Fera
551 Curtobacterium flaccumfaciens pv. flaccumfaciens Seeds Phaseolus vulgaris detection Molecular Extraction DNA RNA, Molecular real time PCR no, yes no
no
2024-12-23 ILVODGB
552 Xanthomonas citri pv. aurantifolii, Xanthomonas citri pv. citri Pure culture identification MALDI-TOF no
no
2024-09-09 NIVIP
553 Tobamovirus maculatessellati / Tomato mottle mosaic virus Seeds Capsicum annuum, Solanum lycopersicum detection and identification Molecular Extraction DNA RNA, Molecular real time RT PCR yes no
no
2023-06-05 Fera
555 Phytoplasma Leaves, Other Catharanthus, Plantae, Vitis detection and identification Molecular Conventional PCR, Molecular Extraction DNA RNA, Molecular HTS, Molecular other, Molecular real time PCR no, yes no, yes
Conventional nested PCR using the primers P1/P7 and R16F2n/R16R2		 no
2022-07-21 NIVIP
556 Xylella fastidiosa Other detection Molecular Extraction DNA RNA, Molecular real time PCR yes yes
Real-time PCR (Harper et al.
2010; erratum 2013)		 no
2024-10-23 LNPVAAL




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