LIST OF VALIDATION DATA


The validation data on tests provided in this EPPO database represent the situation regarding these tests at the moment when the validation was performed and in the conditions of that validation.

Organism Short description Follow EPPO/IPPC diag Methods PDF LabID
Acidovorax citrulli Detection of Acidovorax citrulli by PCR in seeds yes molecular NAKTUINBOUW
Aphelenchoides besseyi Diagnostic Real-time PCR assay for identification and detection of Aphelenchoides besseyi yes molecular CLEAR
Apple proliferation phytoplasma Detection of ‘Candidatus Phytoplasma mali’, ‘Ca. P. pyri’ and ‘Ca. P. prunorum’ by real-time PCR yes extraction
molecular		 NIB-FITO
Beet necrotic yellow vein virus Detection of BNYVV by ELISA in host plant material yes serological LNPVAAL
Bursaphelenchus xylophilus Identification of Bursaphelenchus xylophilus by specifc PCR Castagnone et al. 2005 yes molecular LNPVnema
Bursaphelenchus xylophilus Morphological identification of Bursaphelenchus xylophilus yes morphological LNPVnema
Bursaphelenchus xylophilus Identification of Bursaphelenchus xylophilus by nested PCR (Takeuchi et al. 2005) yes molecular LNPVnema
Bursaphelenchus xylophilus Detection of Bursaphelenchus xylophilus in wood extract with real-time PCR Cao et al. 2005 yes molecular LNPVnema
Bursaphelenchus xylophilus Detection of Bursaphelenchus xylophilus in wood extract with PCR RFLP Burgersmeister et al. 2005 yes molecular LNPVnema
Bursaphelenchus xylophilus Identification of Bursaphelenchus xylophilus by species specific PCR yes molecular LNPVnema
Bursaphelenchus xylophilus Specific PCR Leal et al. 2005 yes molecular LNPVnema
Bursaphelenchus xylophilus Detection of Bursaphelenchus xylophilus in wood extract with real-time PCR François et al. 2007 yes molecular LNPVnema
Bursaphelenchus xylophilus Duplex PCR based identification of Bursaphelenchus xylophilus Jiang et al. 2005 yes molecular LNPVnema
Bursaphelenchus xylophilus Detection of Bursaphelenchus xylophilus in wood extract with real-time PCR Leal et al. 2005 yes molecular LNPVnema
Bursaphelenchus xylophilus detection of Bursaphelenchus xylophilus by real-time PCR yes molecular NIVIP
'Candidatus Liberibacter solanacearum' Diagnostic protocol for the detection and identification of 'Candidatus Liberibacter solanacearum' in carrot seeds (DNA extraction method, real-time PCR and conventional PCR) yes extraction
molecular		 CREA-DC-Roma
'Candidatus Liberibacter solanacearum' Detection of ‘Candidatus Liberibacter solanacearum’ using real-time PCR test yes extraction
molecular		 EVIRAPQL
'Candidatus Liberibacter solanacearum' Analytical sensitivity of the detection of 'Candidatus Liberibacter solanacearum' by real time PCR in carrot seeds using Plant Print diagnòstics kit yes extraction
molecular		 IVIA-B
'Candidatus Liberibacter solanacearum' Detection of 'Candidatus Liberibacter solanacearum' by real time PCR in different types of plant material using Plant Print diagnòstics kit yes extraction
molecular		 IVIA-B
'Candidatus Liberibacter solanacearum' Detection of 'Candidatus Liberibacter solanacearum' by real time PCR in carrot seeds using Plant Print diagnòstics kit yes extraction
molecular		 IVIA-B
Candidatus Liberibacter solanacearum Detection of 'Candidatus Liberibacter solanacearum' by means of the real-time PCR procedure yes extraction
molecular		 ILVODGB
Candidatus Phytoplasma mali Detection of fruit trees phytoplasmas by PCR followed by RFLP analysis yes molecular LNPVAAL
'Candidatus Phytoplasma mali' - Apple proliferation phytoplasma Detection of 'Candidatus Phytoplasma mali' by direct PCR yes extraction
molecular		 CREA-DC-Roma
Candidatus Phytoplasma mali - Apple proliferation phytoplasma Detection of 'Candidatus Phytoplasma mali' by SYBR Green real time PCR yes extraction
molecular		 CREA-DC-Roma
'Candidatus Phytoplasma mali' - Apple proliferation Phytoplasma Detection of 'Candidatus Phytoplasma mali' by direct and nested PCR yes extraction
molecular		 CREA-DC-Roma
Candidatus Phytoplasma prunorum Detection of fruit trees phytoplasmas by PCR followed by RFLP analysis yes molecular LNPVAAL
Candidatus Phytoplasma prunorum - European stone fruit yellows (ESFY) phytoplasma Detection of 'Candidatus Phytoplasma prunorum' by real time PCR yes extraction
molecular		 CREA-DC-Roma
Candidatus Phytoplasma prunorum - European stone fruit yellows (ESFY) phytoplasma Detection of 'Candidatus Phytoplasma prunorumi' by direct and nested PCR yes extraction
molecular		 CREA-DC-Roma
Candidatus Phytoplasma pyri Detection of fruit trees phytoplasmas by PCR followed by RFLP analysis yes molecular LNPVAAL
Chalara fraxinea Detection of Chalara fraxinea by duplex real-time PCR test in planta no molecular LNPVUMAF
Chrysanthemum stem necrosis tospovirus Screening DAS-ELISA test for Chrysanthemum stem necrosis virus yes serological ILVODGB
Chrysanthemum stem necrosis tospovirus Detection of Tospoviruses by RT-PCR in plant material yes extraction
molecular		 LNPVAAL
Clavibacter michiganensis subsp. michiganensis Detection of Clavibacter michiganensis subsp. michiganensis from seed by plating yes isolation NIVIP
Clavibacter michiganensis subsp. michiganensis Identification of Clavibacter michiganensis subsp. michiganensis by IF yes serological NIVIP
Clavibacter michiganensis subsp. michiganensis Detection of Clavibacter michiganensis subsp. michiganensis from symptomatic plant material by plating yes isolation NIVIP
Clavibacter michiganensis subsp. michiganensis Identification of Clavibacter michiganensis subsp. michiganensis by real-time PCR yes molecular NIVIP
Clavibacter sepedonicus Detection of Clavibacter sepedonicus in potato extract by conventional PCR, in a multiplex assay with Ralstonia solanacearum yes molecular IPS2
Columnea latent viroid Validation of a conventional RT-PCR assay for detection and identification of CLVd, PSTVd and TCDVd using primers Vid-FW/RE (verhoeven et al. 2004) yes molecular NIVIP
Cucumber vein yellowing virus Detection of CVYV and CYSDV in host plant material yes molecular LNPVAAL
Cucurbit yellow stunting disorder virus Detection of CVYV and CYSDV in host plant material yes molecular LNPVAAL
Curtobacterium flaccumfaciens pv flaccumfaciens Identification of Curtobacterium flaccumfaciens pv flaccumfaciens by conventional PCR yes molecular NIVIP
Diabrotica virgifera virgifera Morphological identification of D.virgifera in pheromone traps no extraction
morphological		 ILVODGB
Ditylenchus destructor Diagnostic Real-time PCR assays for identification and detection of Ditylenchus dipsaci and D. destructor yes molecular CLEAR
Ditylenchus dipsaci Diagnostic Real-time PCR assays for identification and detection of Ditylenchus dipsaci and D. destructor yes molecular CLEAR
Erwinia amylovora Lateral flow devices for fire blight testing yes serological AGES
Erwinia amylovora Real-time PCR assay targeting chromosomal DNA of Erwinia amylovora. yes molecular AGES
Erwinia amylovora Detection of Erwinia amylovora in plant extract by Conventional PCR, targeting plasmid pEA29 yes extraction
molecular		 FGU VNIIKR
Erwinia amylovora Detection of Erwinia amylovora in plant extract by Real-time PCR, targeting chromosomal DNA yes extraction
molecular		 FGU VNIIKR
Erwinia amylovora Detection of Erwinia amylovora from plant material by Real time PCR yes molecular IVIA-B
Erwinia amylovora Detection of Erwinia amylovora from plant material by Conventional PCR according to Gotsberger, adapted from Obradovic et al. (2007) yes molecular IVIA-B
Erwinia amylovora Detection of Erwinia amylovora from plant material by Conventional PCR according to Taylor et al. (2001) yes molecular IVIA-B
Erwinia amylovora Detection of Erwinia amylovora from plant material by Enrichment-DASI ELISA in King’s B or CCT liquid media yes serological IVIA-B
Erwinia amylovora Extraction of Erwinia amylovora from plant material followed by isolation in CCT medium yes extraction
isolation		 IVIA-B
Erwinia amylovora Detection of Erwinia amylovora from plant material by Conventional PCR aaccording to Stöger et al (2006) yes molecular IVIA-B
Erwinia amylovora Detection of Erwinia amylovora from plant material by immunofluorescence yes serological IVIA-B
Erwinia amylovora Isolation of Erwinia amylovora from plant material in medium King’s B yes isolation IVIA-B
Erwinia amylovora Detection of Erwinia amylovora from plant material by Commercial lateral flow device Pocket Diagnostics yes serological IVIA-B
Erwinia amylovora Detection of E. amylovora by Loop mediated isothermal amplification in shoots and leaves yes molecular IVIA-B
Erwinia amylovora Detection of Erwinia amylovora from plant material by Commercial lateral flow device Ea Agri-Strip yes serological IVIA-B
Erwinia amylovora detection of Erwinia amylovora by isolation on culture medium yes isolation LCF
Erwinia amylovora Real-time PCR detection of Erwinia amylovora using Ams amplicon yes extraction
molecular		 NIB-FITO
European stone fruit yellows phytoplasma Detection of ‘Candidatus Phytoplasma mali’, ‘Ca. P. pyri’ and ‘Ca. P. prunorum’ by real-time PCR yes extraction
molecular		 NIB-FITO
Globodera pallida Diagnostic Real-time PCR assays for identification and detection of Globodera rostochiensis, G. pallida and G. tabacum yes molecular CLEAR
Globodera pallida Identification of potato cyst nematodes using a real-time PCR test yes molecular EVIRAPQL
Globodera pallida Diagnosis of Globodera pallida and Globodera rostochiensis (potato cyst nematodes) using Taqman® real-time PCR yes extraction
molecular		 Fera
Globodera pallida Identification of Globodera pallida and G. rostochiensis by species specific PCR (Vejl 2002) yes molecular LNPVnema
Globodera pallida Species specific identification by conventional PCR test yes molecular LNPVnema
Globodera pallida Identification of Globodera pallida and G. rostochiensis by species specific PCR - Fullaondo 1999 yes molecular LNPVnema
Globodera pallida Molecular identification of Globodera pallida and G. rostochiensis - PCR-RFLP yes molecular LNPVnema
Globodera pallida Qualitative detection of viability and identification of Globodera spp. yes molecular NAK
Globodera rostochiensis Diagnostic Real-time PCR assays for identification and detection of Globodera rostochiensis, G. pallida and G. tabacum yes molecular CLEAR
Globodera rostochiensis Identification of potato cyst nematodes using a real-time PCR test yes molecular EVIRAPQL
Globodera rostochiensis Diagnosis of Globodera pallida and Globodera rostochiensis (potato cyst nematodes) using Taqman® real-time PCR yes extraction
molecular		 Fera
Globodera rostochiensis Species specific identification by conventional PCR test yes molecular LNPVnema
Globodera rostochiensis Molecular identification of Globodera pallida and G. rostochiensis - PCR-RFLP yes molecular LNPVnema
Globodera rostochiensis Identification of Globodera pallida and G. rostochiensis by species specific PCR - Fullaondo 1999 yes molecular LNPVnema
Globodera rostochiensis Identification of Globodera pallida and G. rostochiensis by species specific PCR (Vejl 2002) yes molecular LNPVnema
Globodera rostochiensis Qualitative detection of viability and identification of Globodera spp. yes molecular NAK
Globodera sp. DNA extraction from nematodes juveniles by crushing between slide and coverslip yes molecular LNPVnema
Globodera sp. DNA extraction from nematodes juveniles yes molecular LNPVnema
Globodera tabacum Diagnostic Real-time PCR assays for identification and detection of Globodera rostochiensis, G. pallida and G. tabacum yes molecular CLEAR
Grapevine bois noir phytoplasma Detection of FD and BN by real time PCR yes extraction
molecular		 NIB-FITO
Grapevine flavescence dorée phytoplasma Simoultaneous detection of FD and BN phytoplasmas by multiplex nested-PCR yes molecular EUPH-GRAFDEPI
Grapevine flavescence dorée phytoplasma Detection and identification of Flavescence dorée phytoplasma by direct and nested PCR followed by RFLP yes molecular EUPH-GRAFDEPI
Grapevine flavescence dorée phytoplasma Triplex real time PCR for simultaneous detection of FD and BN phytoplasmas and an internal control (under patent IPADLAB) yes molecular EUPH-GRAFDEPI
Grapevine flavescence dorée phytoplasma Simplex real time PCR for the detection of FD and BN phytoplasmas with an internal control - Hren et al., 2007 yes molecular EUPH-GRAFDEPI
Grapevine flavescence dorée phytoplasma Triplex real-time PCR for simultaneous detection of FD and BN phytoplasmas and an internal control for grapevine. - Pelletier et al., 2009 yes molecular EUPH-GRAFDEPI
Grapevine flavescence dorée phytoplasma Detection of Flavescence dorée phytoplasma by direct universal PCR and nested 16SrV-group specific PCR yes molecular EUPH-GRAFDEPI
Grapevine flavescence dorée phytoplasma Simplex real time PCR for the detection of FD and BN phytoplasmas with an internal control for grapevine - Angelini et al., 2007. yes molecular EUPH-GRAFDEPI
Grapevine flavescence dorée phytoplasma Detection of grapevine phytoplasmas of the 16SrV and 16SrXII-A groups yes molecular LNPVAAL
Grapevine flavescence dorée phytoplasma Detection of FD and BN by real time PCR yes extraction
molecular		 NIB-FITO
Grapevine flavescence dorée phytoplasma Detection of flavescence doree phytoplasma by LAMP in grapevine yes extraction
molecular		 NIB-FITO
Heterodera glycines Identification of Heterodera glycines yes molecular LNPVnema
Heterodera glycines Identification of Heterodera glycines no molecular LNPVnema
Heterodera glycines Identification of Heterodera glycines by PCR-RFLP no molecular LNPVnema
Heterodera glycines Identification of Heterodera glycines by PCR no molecular LNPVnema
Impatiens necrotic spot tospovirus Detection of Tospoviruses by RT-PCR in plant material yes extraction
molecular		 LNPVAAL
Liriomyza huidobrensis Identification of Liriomyza huidobrensis by morphological method no extraction
morphological		 LNPHL
Maize redness phytoplasma Detection of Maize redness phytoplasma by real time PCR yes extraction
molecular		 NIB-FITO
Meloidogyne Detection of Meloidogyne sp. by extraction with automated zonal centrifuge (AZC) from soil and potato tubers, followed by microscopy yes extraction
morphological		 ILVODGB
Meloidogyne chitwoodi Diagnostic Real-time PCR assays for identification and detection of Meloidogyne chitwoodi and M. fallax yes molecular CLEAR
Meloidogyne chitwoodi Identification of Meloidogyne chitwoodi by PCR yes molecular ILVODGB
Meloidogyne chitwoodi Specied specific PCR detection for Meloidogyne chitwoodi, M. fallax and M. hapla yes molecular LNPVnema
Meloidogyne chitwoodi Identification of Meloidogyne chitwoodi and M. fallax by SCAR PCR yes molecular LNPVnema
Meloidogyne chitwoodi Detection of Meloidogyne chitwoodi and M. fallax by PCR RFLP yes molecular LNPVnema
Meloidogyne chitwoodi MeloTuber Test: a real-time TaqMan® PCR-based test to detect the root-knot nematodes Meloidogyne chitwoodi and M. fallax directly in potato tubers, yes molecular NAK
Meloidogyne enterolobii Identification of meloidogyne enterolobii by SCAR PCR yes molecular LNPVnema
Meloidogyne fallax Diagnostic Real-time PCR assays for identification and detection of Meloidogyne chitwoodi and M. fallax yes molecular CLEAR
Meloidogyne fallax Specied specific PCR detection for Meloidogyne chitwoodi, M. fallax and M. hapla yes molecular LNPVnema
Meloidogyne fallax Identification of Meloidogyne chitwoodi and M. fallax by SCAR PCR yes molecular LNPVnema
Meloidogyne fallax Detection of Meloidogyne chitwoodi and M. fallax by PCR RFLP yes molecular LNPVnema
Meloidogyne fallax MeloTuber Test: a real-time TaqMan® PCR-based test to detect the root-knot nematodes Meloidogyne chitwoodi and M. fallax directly in potato tubers, yes molecular NAK
Mexican papita viroid Validation of a conventional RT-PCR test for detection and identification of PSTVd, TCDVd, MPVd and TPMVd using primers 2H1/3H1 described by Shamoul et al. (1997) yes molecular NIVIP
Monilia fructicola Detection of Monilia fructicola by PCR in plant material no molecular LNPVUMAF
Monilinia fructicola Detection of Monilinia fructicola by multiplex PCR yes molecular CREA-DC-Roma
Monilinia fructicola detection of Monilinia fructicola in fruits by real-time PCR yes molecular NIVIP
Nacobbus aberrans PCR detection of Nacobbus aberrans no molecular LNPVnema
Nepovirus Detection of Nepovirus by RT-PCR yes extraction
molecular		 LNPVAAL
Pantoea stewartii subsp. stewartii Conventional PCR (AGES primers) from corn seed macerate yes molecular EUPH-PANTOEA
Pantoea stewartii subsp. stewartii Immunofluorescence from corn seed macerate yes serological EUPH-PANTOEA
Pantoea stewartii subsp. stewartii Real-time PCR from corn seed macerate yes extraction
molecular		 EUPH-PANTOEA
Pantoea stewartii subsp. stewartii Conventional PCR (Coplin primers) from corn seed macerate yes molecular EUPH-PANTOEA
Pantoea stewartii subsp. stewartii detection of Pantoea stewartii subsp.stewartii using a FLASH PCR detection Kit yes extraction
molecular		 FGU VNIIKR
Pantoea stewartii subsp. stewartii Detection of Pantoea stewartii subsp. stewartii in plant and seed extract by conventional PCR yes molecular IPS2
Pear decline phytoplasma Detection of ‘Candidatus Phytoplasma mali’, ‘Ca. P. pyri’ and ‘Ca. P. prunorum’ by real-time PCR yes extraction
molecular		 NIB-FITO
Pepino mosaic virus Detection of PepMV by RT-Q PCR in tomato seed and leaves no extraction
molecular		 LNPVAAL
Phyllosticta citricarpa Real Time PCR for the identification of Phyllosticta citricarpa (van Gent-Pelzer et al., 2007) yes molecular CREA-DC-Roma
Phyllosticta citricarpa Detection of Phyllosticta citricarpa by PCR (Bonants et al. 2003) yes molecular CREA-DC-Roma
Phytophthora kernoviae Detection of Phytophthora kernoviae by plating infected plant material and morfological evaluation the culture yes extraction
morphological		 ILVODGB
Phytophthora ramorum Detection of Phytophthora ramorum by plating infected plant material and morfological evaluation the culture no extraction
morphological		 ILVODGB
Plum pox virus Detection of Plum pox virus by direct and indirect ELISA yes extraction
serological		 CREA-DC-Roma
Plum pox virus Detection of Plum pox virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP) yes extraction
molecular		 CREA-DC-Roma
Plum pox virus Detection of Plum pox virus in plant extract by FLASH-PCR yes extraction
molecular		 FGU VNIIKR
Plum pox virus Detection of PPV by DAS ELISA and real-time PCR in leaves of Prunus yes molecular
serological		 ILVODGB
Plum pox virus detection of PPV, PNRV and PDV by DAS-ELISA yes serological IPS2
Plum pox virus detection of plum pox virus using DAS-ELISA no serological SPAOLO
Pospiviroid Detection of pospiviroids by means of one-step real-time PCR method yes molecular ILVODGB
Pospiviroid Detection of pospiviroid in potato leaves by real-time RT-PCR no molecular NAK
Pospiviroid Detection of pospiviroids by realtime RT-PCR on tomato and pepper seeds, i.e. CEVd, CLVd, PCFVd, TASVd, TCDVd, TPMVd yes extraction
molecular		 NAKTUINBOUW
Pospiviroid Real-time RT-PCR (TaqMan RT-PCR) for pospivirods in leaves of horticultural crops yes molecular NAKTUINBOUW
Pospiviroid Development and validation of a real-time RT-PCR assay for generic detection of Pospiviroids yes molecular NIVIP
Pospiviroid Validation of a conventional RT-PCR assay for detection and preliminary identification of pospiviroids (expect CLVd) by Pospi1-FW/Pospi1-RE yes molecular NIVIP
Potato spindle tuber viroid detection of PSTVd by RT-PCR and real-time RT-PCR yes molecular IPS2
Potato spindle tuber viroid Detection of PSTVd ​​in potato leaf using Boonham real-time RT -PCR. version 1 yes molecular NAK
Potato spindle tuber viroid Real-time RT-PCR (TaqMan RT-PCR) for Potato spindle tuber viroid (PSTVd) and/or Tomato chlorotic dwarf viroid (TCDVd) in leaf material of horticultural crops yes molecular NAKTUINBOUW
Potato spindle tuber viroid Detection of Potato spindle tuber viroid (PSTVd) and/or Tomato chlorotic dwarf viroid (TCDVd) in tomato seed with real-time RT-PCR (TaqMan RT-PCR). yes molecular NAKTUINBOUW
Potato spindle tuber viroid Validation of a conventional RT-PCR assay for detection and identification of CLVd, PSTVd and TCDVd using primers Vid-FW/RE (verhoeven et al. 2004) yes molecular NIVIP
Potato spindle tuber viroid Validation of a conventional RT-PCR test for detection and identification of PSTVd, TCDVd, MPVd and TPMVd using primers 2H1/3H1 described by Shamoul et al. (1997) yes molecular NIVIP
Prune dwarf virus detection of PPV, PNRV and PDV by DAS-ELISA yes serological IPS2
Prunus necrotic ringspot virus detection of PPV, PNRV and PDV by DAS-ELISA yes serological IPS2
Pseudomonas syringae pv. actinidiae Detection of Pseudomonas syringae pv. actinidiae by isolation and PCR assays in plant material and bacterial cultures yes extraction
molecular
isolation
fingerprint
morphological		 CREA-DC-Roma
Ralstonia solanacearum Detection of Ralstonia solanacearum by egl LAMP in plant material yes molecular NIB-FITO
Ralstonia solanacearum Detection of R. solanacearum by IF in Pelargonium sp. yes serological NIVIP
Ralstonia solanacearum Isolation of Ralstonia solanacearum on SMSA yes isolation NIVIP
Stolbur phytoplasma Detection of grapevine phytoplasmas of the 16SrV and 16SrXII-A groups yes molecular LNPVAAL
Thrips palmi Morphological identification of Thrips palmi no extraction
morphological		 ILVODGB
Tomato chlorosis virus Detection of ToCV by RT-PCR in tomato leaves no extraction
molecular		 LNPVAAL
Tomato chlorotic dwarf viroid Detection of Potato spindle tuber viroid (PSTVd) and/or Tomato chlorotic dwarf viroid (TCDVd) in tomato seed with real-time RT-PCR (TaqMan RT-PCR). yes molecular NAKTUINBOUW
Tomato chlorotic dwarf viroid Real-time RT-PCR (TaqMan RT-PCR) for Potato spindle tuber viroid (PSTVd) and/or Tomato chlorotic dwarf viroid (TCDVd) in leaf material of horticultural crops yes molecular NAKTUINBOUW
Tomato chlorotic dwarf viroid Validation of a conventional RT-PCR assay for detection and identification of CLVd, PSTVd and TCDVd using primers Vid-FW/RE (verhoeven et al. 2004) yes molecular NIVIP
Tomato chlorotic dwarf viroid Validation of a conventional RT-PCR test for detection and identification of PSTVd, TCDVd, MPVd and TPMVd using primers 2H1/3H1 described by Shamoul et al. (1997) yes molecular NIVIP
Tomato planta macho viroid Validation of a conventional RT-PCR test for detection and identification of PSTVd, TCDVd, MPVd and TPMVd using primers 2H1/3H1 described by Shamoul et al. (1997) yes molecular NIVIP
Tomato spotted wilt tospovirus DAS-ELISA (screening) and one-step real-time PCR test for Tomato spotted wilt virus yes molecular
serological		 ILVODGB
Tomato spotted wilt tospovirus Detection of Tospoviruses by RT-PCR in plant material yes extraction
molecular		 LNPVAAL
Tomato yellow leaf curl virus Screening TAS-ELISA test for Tomato yellow leaf curl virus - Antibody set ADGEN/NEOGEN 1072-25 yes molecular
serological		 ILVODGB
Tospoviruses Detection of Tospoviruses by RT-PCR in plant material yes extraction
molecular		 LNPVAAL
Xanthomonas axonopodis pv. dieffenbachiae Detection of Xanthomonas axonopodis pv. dieffenbachiae by DAS-ELISA in leaves and pure culture no extraction
serological		 LNPVRAPT
Xanthomonas axonopodis pv. dieffenbachiae Detection of Xanthomonas axonopodis pv. dieffenbachiae by IF in leaves and pure culture no extraction
serological		 LNPVRAPT
Xanthomonas axonopodis pv. dieffenbachiae Detection of Xanthomonas axonopodis pv. dieffenbachiae by nested-PCR in leaves and pure culture no extraction
molecular		 LNPVRAPT
Xanthomonas euvesicatoria Duplex-PCR tests for the identification X. euvesicatoria, X. vesicatoria, X. gardneri and X. perforans. yes molecular NIVIP
Xanthomonas euvesicatoria Isolation of Xanthomonas sp. from tomato and pepper seeds yes extraction
isolation		 NIVIP
Xanthomonas fragariae Real-time PCR for detection and identification purposes of Xanthomonas fragariae yes molecular NIVIP
Xanthomonas gardneri Isolation of Xanthomonas sp. from tomato and pepper seeds yes extraction
isolation		 NIVIP
Xanthomonas gardneri Duplex-PCR tests for the identification X. euvesicatoria, X. vesicatoria, X. gardneri and X. perforans. yes molecular NIVIP
Xanthomonas perforans Duplex-PCR tests for the identification X. euvesicatoria, X. vesicatoria, X. gardneri and X. perforans. yes molecular NIVIP
Xanthomonas perforans Isolation of Xanthomonas sp. from tomato and pepper seeds yes extraction
isolation		 NIVIP
Xanthomonas vesicatoria Isolation of Xanthomonas sp. from tomato and pepper seeds yes extraction
isolation		 NIVIP
Xanthomonas vesicatoria Duplex-PCR tests for the identification X. euvesicatoria, X. vesicatoria, X. gardneri and X. perforans. yes molecular NIVIP
Xylella fastidiosa Detection of Xylella fastidiosa in perennial host species by LAMP-PCR no extraction
molecular		 ISPP-CNR
Xylella fastidiosa detection of Xylella fastidiosa in composite samples of polygala myrtifolia no extraction
molecular		 ISPP-CNR
Xylella fastidiosa Detection of Xylella fastidiosa in perennial host species by Real time PCR (Harper et al. 2010 erratum 2013) no extraction
molecular		 ISPP-CNR
Xylella fastidiosa detection of Xylella fastidiosa in composite olive samples no extraction
molecular		 ISPP-CNR
Xylella fastidiosa detection of Xylella fastidiosa in composite samples of lavander no extraction
molecular		 ISPP-CNR
Xylella fastidiosa Detection of Xylella fastidiosa in perennial host species by ELISA no serological ISPP-CNR
Xylella fastidiosa Detection of Xylella fastidiosa in perennial host species by Real time PCR Francis et al., 2006 modified using SYBR green no extraction
molecular		 ISPP-CNR
Xylella fastidiosa detection of Xylella fastidiosa in composite samples of herbaceous hosts no extraction
molecular		 ISPP-CNR
Xylella fastidiosa detection of Xylella fastidiosa in composite samples of herbaceous hosts no extraction
molecular		 ISPP-CNR
Xylella fastidiosa detection of Xylella fastidiosa in composite samples of oleander no extraction
molecular		 ISPP-CNR
Xylella fastidiosa detection of Xylella fastidiosa in composite samples of cherry no extraction
molecular		 ISPP-CNR
Xylella fastidiosa Detection of Xylella fastidiosa in perennial host species by Real time PCR no extraction
molecular		 ISPP-CNR
Xylella fastidiosa Detection of Xylella fastidiosa in perennial host species by PCR yes extraction
molecular		 ISPP-CNR
Xylella fastidiosa Detection of Xylella fastidiosa by conventional PCR in plant material yes extraction
molecular		 LNPVAAL
Xylella fastidiosa Detection of Xylella fastidiosa by real-time PCR in plant material (Harper et al., 2010, Erratum 2013) yes extraction
molecular		 LNPVAAL
Xylella fastidiosa Detection of Xylella fastidiosa by immunofluorescence in plant material yes serological LNPVAAL
Xylella fastidiosa Détection de Xylella fastidiosa par PCR en temps réel sur végétal yes molecular LNPVAAL
Xylella fastidiosa Detection of Xylella fastidiosa by real-time PCR in insects yes extraction
molecular		 LNPVAAL
Xylella fastidiosa Detection of Xylella fastidiosa by real-time PCR (Schaad et al., 2002) in plant material yes molecular NIB-FITO
Xylella fastidiosa Detection of Xylella fastidiosa by real-time PCR (Harper et al., 2010, erratum 2013) in plant material yes molecular NIB-FITO
Xylella fastidiosa Detection of Xylella fastidiosa by real-time PCR (Francis et al., 2006) in plant material yes molecular NIB-FITO
Xylella fastidiosa Detection of Xylella fastidiosa by real-time PCR in plant material no molecular NIB-FITO
Xylella fastidiosa Validation of the real-time PCR for the detection of Xylella fastidiosa (Ouyang et al., 2013) in comparison with the real time PCR of Harper et al. (2010; erratum 2013). yes extraction
molecular		 NIVIP
Xylella fastidiosa subsp. pauca Detection of Xylella fastidiosa subsp. pauca ceppo CoDiRo by LAMP-PCR from plant olive crude extracts yes extraction
molecular		 CREA-DC-Roma
Xylella fastidiosa subsp. pauca Detection of Xylella fastidiosa subsp. pauca ceppo CoDiRo by LAMP-PCR from DNA extracted from olive leaves and petioles yes extraction
molecular		 CREA-DC-Roma
Xylella fastidiosa subsp. pauca Detection of Xylella fastidiosa subsp. pauca ceppo CoDiRo in plant olive extracts by real time PCR yes extraction
molecular		 CREA-DC-Roma
Xylella fastidiosa subsp. pauca Detection of Xylella fastidiosa subsp. pauca ceppo CoDiRo from plant olive extracts by ELISA test yes extraction
serological		 CREA-DC-Roma
Xylella fastidiosa subsp. pauca strain CoDiRO Detection of Xylella fastidiosa subsp. pauca ceppo CoDiRo from plant olive extracts by Conventional PCR according to Minsavage et al. (1994) yes extraction
molecular		 CREA-DC-Roma

EU H2020


This project has received funding from the European Union's Horizon 2020 research
and innovation programme under grant agreement n. 773139. The content of this website represents the views of the author only and is his/her sole responsibility. It cannot be considered to reflect the views of the European Commission and/or the Research Executive Agency or any other body of the European Union. The European Commission and the Agency do not accept any responsibility for use that may be made of the information it contains.